OBJECTIVE:To study process conditions and parameters of purification of total gingko flavones-glycoides by macroporous adsorptive resin. METHODS:To select the optimal resin used to separate glycoides among 6 different macroporous resins by static adsorption and desorption and investigate the adsorption property and optimal elution parameters of the best resin by dynamic adsorption. RESUTS:The results show that HPD-450 macroporous resin is the best resin used for separating glycosides,and the optimal technological conditions were as fol ows: the sample amount 6.5 times the column volumes, sample flow velocity 5 mL/min, pH value 4.0, eluting with 400 mL of 85% ethanol at the eluting velocity of 4mL/min. CONCLUSIONS:It turned out that total flavonoids purity more than 26% of ginkgo biloba extract according to the optimum.
ginkgo biloba extractmacro-porous resinsflavonesorthogonal test
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