首页|过氧化氢通过激活氧化应激诱导成骨细胞凋亡

过氧化氢通过激活氧化应激诱导成骨细胞凋亡

hydrogen peroxide induce osteoblastic cell apoptosis

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目的：探讨氧化应激状态诱导成骨细胞系 MC3T3-E1凋亡中线粒体途径的作用。方法：采用 MTT 法检测不同浓度过氧化氢处理后 MC3T3-E1的增殖情况；Annexin V-FITC/PI流式细胞学检测细胞凋亡；JC-1流式细胞学检测线粒体膜电位；DCFH-DA流式检测ROS活性。结果：MC3T3-E1过氧化氢处理后，细胞增殖明显抑制呈剂量效应关系，ROS 活性和凋亡增加，线粒体膜电位降低。结论：过氧化氢可以增加细胞内 ROS 水平，通过激活线粒体途径诱导细胞凋亡。
Objective:To investigate the effect mitochondria signal patway on the apoptosis of MC3T3-E1 induced by hydrogen peroxide. Methods:MC3T3-E1 cells were treated with different doses of hydrogen peroxide. MTT was used to exam the cellinhibition rate. We decected the apoptosis rate, JC-1 the leve of ROS and the expression of Bax in mitochondria. Results:The apoptosis rate and the leve of ROS were increased by hydrogen peroxide, mitochondria membrane potential was deceased. Conclusion:Hydrogen peroxide can induce MC3T3-E1 cellapoptosis through mitochondria signal pathway.

刘红、候宝贤、郭宝磊、宗丹、关和宇、潘梁、王鹏、裴卓斐

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沈阳市骨科医院 辽宁 沈阳 110001

2013

中国保健营养(中旬刊)
全国卫生产业企业管理协会

中国保健营养(中旬刊)

ISSN：1004-7484
年,卷(期)：2013.(11)
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