首页|ITS2序列鉴定临床分离丝状真菌

ITS2序列鉴定临床分离丝状真菌

Identification of filamentous fungi from clinical specimens by sequence analysis of the Internal Transcribed Spacer Region 2

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目的：建立以ITS2为靶序列鉴定临床分离丝状真菌的分子生物诊断方法。方法：利用通用引物扩增真菌核糖体转录间隔区2（ITS2），对PCR产物进行测序，在GenBank中对测序结果进行Blastn等分析。结果：9株真菌测序结果与表型鉴定符合，鉴定出7株临床分离丝状真菌。结论：ITS2测序可以作为临床丝状真菌的分子生物学诊断方法之一。
Objective:To establish a method of sequencing of internal transcribed spacer 2 (ITS2) for identification of filamentous fungi from clinical specimens. Methods:Amplification and sequencing of internal transcribed spacer 2 (ITS2) region of fungi were performed using universal primers. Searches of current nucleotide databases in GenBank were carried out with the BLAST algorithm. Results:9 clinical common fungal isolates were accurately identified . 6 clinical filamentous fungi isolates were detected. Conclusions:ITS2 sequencing for ilamentous fungi is an excellent method.

filamentous fungiinternal transcribed spacerITSsequencing

吴奎海、马均宝、崔东岚

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广东省佛山市第一人民医院检验科 广东 佛山 528000

丝状真菌 内部转录间隔区 测序

2013

中国保健营养(中旬刊)
全国卫生产业企业管理协会

中国保健营养(中旬刊)

ISSN：1004-7484
年,卷(期)：2013.(11)
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