Effect of marine collagen peptides on cutaneous wound healing in rats
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目的：观察海洋胶原肽(marine col agen peptides, MCPs)对大鼠术后伤口愈合的影响，以期为术后营养支持提供实验室依据。方法：取120只体重为230-250g的SD大鼠，分为5组，每组24只，包括空白对照组和4个MCPs组（分别为0.667g/kg.bw、2.0g/kg.bw、6.0g/kg.bw和12g/kg.bw），空白对照组给予等体积溶剂灌胃，进行背部皮肤深达腹腔的切口手术，分别于术后第3、7、14天处死动物，各时间点8只动物。术后每日观察伤口的愈合情况，进行HE染色、Masson染色，皮肤切口抗张力强度测定和羟脯氨酸测定。结果：MCPs 能够促进伤口恢复，增强皮肤切口的胶原纤维表达和抗张力强度。结论： MCPs 可以通过改善大鼠术后的营养状况进而促进伤口愈合。
OBJECTIVE:To investigate the effects of marine col agen peptides (MCPs) on wound healing in rats. METHODS:120 healthy male SD rats were divided into 5 groups, which were normal control (NC) group and 0.667, 2.0, 6.0, 12g/kg.bw MCPs groups. MCPs were oral y administered by gavage to rats in MCPs group from postoperation, and the control rats were given vehicle only. 120 rats underwent 2-cm length surgical ful-thickness incision (including the panniculus muscle and the peritoneum) simulating upper medial laparatomy and were chosed with surgical sutures. Necropsy was performed on postoperative day 3, 7 and 14. Eight animals per group were kil ed at each examined time points after incision. Wound strips were removed along incision including 5 mm from the edges and were used as experimental samples for histological analysis, tensile strength and hydroxyproline measurement. RESULTS:MCPs accelerated the wound healing, tensile strength and hydroxyproline expression. CONCLUSION:MCPs can enhance cutaneous wound healing in rats via nutrition support.
marine col agen peptidesnutrition supportsurgerywound healing
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