Objective:To explore the effect of IL-22 on the function of spleen and mesenteric lymph nodes from graft-versus-host disease mice. Methods:BALB/c and C57BL/6 mice (6-8W) were used as recipients and donors respectively to established GVHD model. GVHD mice was divided into GVHD+PBS group and GVHD+IL-22 group. The number of splenic cells and lymphcytes were detected on day 28. Percentages of IFN-γ-producing T cells were investigated by Flow cytometry. Results:The total number of splenic cells and lymphcytes in GVHD+IL-22 mice were lower than that in GVHD+PBS mice, while necrosis areas were detected in spleen from GVHD+IL-22 mice. Although there was no effect to the secretion of IFN-γin splenic CD4+and CD8+T cells, the percentage of IFN-γ+CD4+T cell(Th1)and IFN-γ+CD8+T cell(Tc1) in lymph nodes increased by IL-22 injection. Conclusion:IL-22 decrease the total number of T cells, but partly increased the IFN-γ-producing T cellability of GVHD mice.
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