Objective:Explore the value of colposcopy with cervical biopsy in the diagnosis of cervical disease.Methods:1088 cases were examed by colposcopy in the gynecological clinic of Affiliated Hospital of Jining Medical University, from June 2012 to June 2013, the results of colposcopy was analyzed by epidemilogical method.Results: 325 cervical biopsy samples were colected ,and compared with the pathological diagnosis, the colposcopy diagnosis accordance rate of cervicitis、CINI、CINII、CINIII is 65.12%、74.42%、83.33%、85.71%.Conclusion:Diagnose accordance rate of colposcopy and pathological diagnosis were higher, which had higher authenticity. it is helpful for early detection of cervical cancer, cervical intraepithelial neoplasia ,and it has a very high diagnostic value.