普罗布考对环磷酰胺致肝损伤的保护作用
Protective Effect and Mechanism of Probucol in the Hepatic Injury of Cyclophosphamide-induced rats
摘要
目的：研究普罗布考对环磷酰胺肝损伤大鼠肝功能的保护作用和其氧化应激的机制。方法：测定普罗布考在不同浓度下对环磷酰胺作用下细胞的增殖活性的影响。将50只大鼠随机分为5组，空白对照组、模型对照组，普罗布考低、中、高（200、400、800mg·kg-1）剂量组，腹腔注射100 mg·kg-1环磷酰胺,每天一次,持续5天,制备环磷酰胺肝损伤模型，10天后检测大鼠血清ALT、AST及肝组织各氧化指标，同时取肝组织标本观察病理学改变。结果：用普罗布考孵育能够明显提高环磷酰胺作用下HL7702细胞存活率；与模型对照组相比，普罗布考组血清ALT、AST明显下降（P＜0.05），肝组织中NO含量、NOS、GSH和SOD活性明显上升、MDA含量明显下降（P＜0.05），肝组织病理损伤减轻。结论：普罗布考能降低环磷酰胺所致大鼠肾脏损伤具有保护作用,起保护作用可能与提高抗氧化应激损伤有关。
Abstract
Aim To observe the effect of different dosages of probucol on the oxidative stress in the hepatic injury of cyclophosphamide-induced rats.Methods:The hepatic injury models were established by ip injection of cyclophosphamide.Al the rats were randomly divided into folowing groups: normal control rats ,model control,different dosages of Probucol treated hepatic injury rats. After 10 days, ALT、ASTwere detected and the liver tissue was acquired for measuring MDA、SOD、GSH-Px、NO and NOS.Result:Compared with model group, ALT、AST and renal concentration of MDA of probucol 800mg·kg-1group decreased significantly(P<0.05), while the activity of SOD、NO、NOS and GSH-Px increased significantly (P<0.05) .Histopathology of mice showed probucol can improve hepatic injury.Conclusion:Probucol can inhibit the oxidative stress in the hepatic injury of cyclophosphamide-induced rats.
关键词
普罗布考/环磷酰胺/肝损伤/氧化应激Key words
hepatic injury/oxidative stress/probucol引用本文复制引用
出版年
2014
NETL
NSTL
万方数据