Objective: Comparing different ways of isolation and purify astrocytes from mouse cerebral tissue to Learn the effect of astrocytes for future study of their functions in the brain.Methods: To acquire purified astrocytes by two usualy used cultural methods: after primary culture, subculture for three times or shanken overnight twice.Results: The purity of primary culture was the lowest ,with the method of being shanken over night twice highly expressed GFAP, Immunofluorescence staining with anti-mouse GFAP was brighter. Astrocytes purified by being subcultured for three times over 97% purity. The expression of by GFAP low.Conclusion: With the subcultural method we can acquire the purer astrocytes in vitro, which the expression of by GFAP lower, and can best reflect their functions in the brain. It’s an optimal method to acquire the astrocytes for future study of their functions in vivo.
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