作者信息
- 1. 青海省贵德县人民医院检验科 811700
- 折叠
摘要
目的：分析化学发光法（微粒子酶免法）与酶联免疫吸附实验（ELISA）及胶体金免疫沉析法检测乙肝表面抗原的差异性。方法：利用雅培i2000化学发光分析仪测定病人标本，记录乙肝表面抗原的定量结果，将同样的标本用酶联免疫吸附实验及胶体金检测，记录检测结果，经统计学处理，对三种方法的测定结果进行比较。结果：三种方法测定乙肝表面抗原的结果存在差异，化学发光法的阳性检出率较ELISA法及胶体金免疫沉析法高。结论：对ELISA法及胶体金免疫沉析法检测乙肝表面抗原处于临界状态的标本，应使用化学发光法检测，以提高结果的准确性，更好的为临床提供诊断依据。
Abstract
Analysis of chemiluminescence (microparticle enzyme immunoassay method) and enzyme-linked immunosorbent assay (ELISA) and immunoprecipitation, analysis of coloidal gold was detected difference between hepatitis B surface antigen.Methods:Abbot i2000 using chemiluminescence analyzer patient specimens. Record the results of the quantitative hepatitis B surface antigen, Wil have the same specimens by enzyme-linked immunosorbent assay and coloidal gold detection,Record test results, Statistical analysis,Determination of the three methods were compared.Results:Three Methods for measuring the results of hepatitis B surface antigen differences,Chemiluminescence than the positive rate of ELISA ,and immunogold method with high precipitation.Conclusion:On ELISA, and immune precipitation of coloidal gold assay of hepatitis B surface antigen in the critical state of the specimens,Chemiluminescence detection should be used to improve the accuracy of the results,the beter the basis for the clinical diagnosis.
关键词
乙肝表面抗原/微粒子酶免法/ELISA胶体金免疫沉析法Key words
Hepatitis B surface antigen/Microparticle enzyme immunoassayl/Method ELISA Immungold precipitation method引用本文复制引用
出版年
2014
NETL
NSTL
万方数据