To develop a method of safety assessment for Clove essential oil.Methods:the content of Clove essential oil was determined by GC with eugenol as a quantitative indicator and its safety was evaluated by the use of acute skin irritation test, multiple skin irritation and eye irritation test.Results:The linearity of eugenol content was good when its concentration was in the range between 0.0997~1.9940 mg/ml, the average rate of recovery was 99.01%, RSD was 1.89%(n=6). With regard to acute skin irritation test and eye irritation test, Clove essential oil was nonirritant at a concentration of 50%. In the multiple skin irritation, Clove essential oil was mild irritant at concentrations of both 75% and 100%. Conclusion:The GC method is fast, simple, accurate and suitable for quality control of Clove essential oil. Clove essential oil is mild irritant for eyes and skin of animal.
Clove essential oilEugenolSafety assessmentDetermination of content
NETL
NSTL
万方数据
