丁香精油的安全性评价
Safety Assessment of Clove Essential Oil
李小平 1王彬2
作者信息
- 1. 海南省药物研究所 海南 海口 570216
- 2. 海南省实验动物中心
- 折叠
摘要
目的：建立丁香精油的安全性评价方法。方法：以丁香酚为指标采用气相色谱法对丁香精油进行含量测定，并分别从急性皮肤刺激试验，多次皮肤刺激试验，眼刺激试验几个方面对其安全性进行分析。结果：丁香酚在0.0997～1.9940 mg/ml 浓度范围内具有良好的线性关系，平均回收率为99.01%，RSD为1.89%（n=6）。急性皮肤刺激试验和眼刺激试验在50%的浓度范围内，丁香精油强度显示为无刺激，多次皮肤刺激试验中，丁香精油浓度在75%和100%时属于轻度刺激性。结论：气相色谱法灵敏、简便,结果准确可靠,可作为控制丁香精油质量的方法。丁香精油对动物皮肤和眼睛的刺激性较小。
Abstract
To develop a method of safety assessment for Clove essential oil.Methods:the content of Clove essential oil was determined by GC with eugenol as a quantitative indicator and its safety was evaluated by the use of acute skin irritation test, multiple skin irritation and eye irritation test.Results:The linearity of eugenol content was good when its concentration was in the range between 0.0997~1.9940 mg/ml, the average rate of recovery was 99.01%, RSD was 1.89%(n=6). With regard to acute skin irritation test and eye irritation test, Clove essential oil was nonirritant at a concentration of 50%. In the multiple skin irritation, Clove essential oil was mild irritant at concentrations of both 75% and 100%. Conclusion:The GC method is fast, simple, accurate and suitable for quality control of Clove essential oil. Clove essential oil is mild irritant for eyes and skin of animal.
关键词
丁香精油/丁香酚/安全性评价/含量测定Key words
Clove essential oil/Eugenol/Safety assessment/Determination of content引用本文复制引用
出版年
2014
NETL
NSTL
万方数据