摘要
萝卜硫素是从十字花科蔬菜中提取的多功能物质，研究已证实其具有抗癌、抗氧化等功效。肺癌是恶性程度高、具有转移倾向的恶性肿瘤，萝卜硫素抗肺癌的机制尚不是十分清楚。本研究通过CCK-8和transwel侵袭实验分析初步判断萝卜硫素对A549肺癌细胞活性和转移侵袭的影响，计算体外干预A549的IC50，流式细胞学分析IC50浓度萝卜硫素对细胞周期和凋亡的影响，电泳分析DNA片段化改变。结果显示A549细胞活性对萝卜硫素剂量依赖性下降，萝卜硫素作用于A549细胞的IC50为3μg/mL,该浓度的萝卜硫素抑制了A549细胞的转移侵袭，阻滞细胞于G2/M期，诱导细胞凋亡并使DNA发生片段化变化，下调TGF-β和NF-κB的表达。结果提示萝卜硫素可以抑制肺癌细胞的生长和侵袭，抑制炎症蛋白的表达，是肺癌辅助治疗的新途径。
Abstract
Sulforaphane was a multifunction compound derived from brassicaceous vegetable such as broccoli, reports showed that Sulforaphane provided with effection of antitumor and antioxidant. Lung cancer is an aggressive malignancy with a tendency of early distant metastases, the antitumor function of sulforaphane was corroborated by numerous lines of evidence, but the anticancer mechanism of this compound has not been wel obsvered. In this work, we analyzed vitality and invasion of A549 cels treated with sulforaphane by cellcounting kit (CCK8) and transwel, then measure the half maximal (50%) inhibitory concentration (IC50) of sulforaphane for A549 cels. The cels cycle, apoptosis and DNA fragment were analyzed using Flow Cytometry Analysis and agarose electrophoresis, TGF-βand NF-κB were analyzed by western blot after treatment with 3μg/mL sulforaphane. Results showed that A549 cels proliferate and invade were inhibited by sulforaphane with a dose-dependent manner, IC50 of sulforaphane was 3μg/mL, and the cellcycle were arrested at G2/M phase. 3μg/mL sulforaphane induced apoptosis , DNA fragment, decreased the expression of TGF-βand NF-κB in A549 cels. Our results pointed out that sulforaphane inhibited proliferation and invasion of lung cancer A549 cels in vitro, decreased the expression of inflammation proteins, maybe a novel chemotherapy for lung cancer.
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