摘要
目的：研究不同浓度的新疆黑蜂胶乙醇提取物对 Balb/c 小鼠淋巴细胞的增殖作用。方法新疆黑蜂胶经乙醇提取混合物，做倍比稀释。Balb/c小鼠脾淋巴细胞分别加入500μg/mL及倍比稀释至15．6μg/mL的黑蜂胶共6个测定浓度，同时设脂多糖对照组、刀豆蛋白A对照组和空白对照组。采用CCK-8测定不同浓度黑蜂胶在体外刺激脾淋巴细胞后的OD450值。结果500μg/mL至62．5μg/mL的黑蜂胶刺激小鼠淋巴细胞增殖的活性显著高于空白对照组（P＜0．05），但低于ConA对照组（P＜0．05）。黑蜂胶的免疫激活作用与其药物浓度呈正相关（r＝0．794，P＜0．05）。结论新疆黑蜂胶可非特异性的促进淋巴细胞显著增殖。
Abstract
Objective To study the different concentrations of Xinjiang black propolis extracted by ethanol stimulate the spleen lymphocyte proliferation of Balb /c mice in vitro.Method Xinjiang black propolis did times dilution from 500μg/mL to 1.95μg/mL in each well,add ConA,LPS and cell buffer fluid,all wells culture with Balb/c mice spleen lymphocytes for 48 hours,using CCK-8 method to detect OD 450 value.Result Compared with control groups,Xinjiang black propolis was shown a stronger activity of lymphocyte proliferation at the concentration from 500ug/mL to 62.5ug/mL than blank control;however,its activity was weaker than ConA control. And its immunological functions were shown a positive correlation with its concentrations .Conclusion Xinjiang black propolis can promote lymphocyte proliferation signif-icantly as a nonspecific stimulator .
基金项目
立项课题全国大学生创新实验设计项目()
新疆医科大学大学生创新性实验计划项目八期课题(CX2013004)
NETL
NSTL
万方数据