Objective ： To study the singniticance of interleukin-8（IL-8）,p53mRNA and Bcl-2mRNA in myocardial infarction reperfusion（MIR） injury. Methods：Build up the big rat MIR the model, ising divided into the matched control and experimenting the group, after infarction 30 mins, then reperfusion to note 2,4,8,12,24,48 h, each time orders to sentence to death the animal respectively, taking the blood back up. the contents of IL-8,p53mRNA and Bcl-2mRNA were examined by ELISA, in situ hybridization and TEUNL techniques. Results：See from MIR time window, the IL-8 starts go up namely in the 2h of IR, the 12h reaches the high peak.The p53 starts go up in the 4h, the 24h reaches the high peak.The Bcl-2 starts go up in the 8h to had been keep on till the 48h.Early the IL-8 expresses in the p53, the nights Bcl-2s express in the p53, the myocardial apoptosis with the p53mRNA the expression time mutually.Conclusion： In the serum the IL-8 increase high lay to lead the organization in the p53 of high expression, repressed the function of the Bcl-2, played an important role in MIR hurt.
myocardial infarction reperfusion injury(MIRI) IL-8 p53mRNA Bcl-2mRNA rat
NETL
NSTL
万方数据
维普
