SSR polymorphism of 11 new strains of Sorghum-Sudangrass was analyzed. Genomic DNAs of Sorghum-Sudangrass were amplified with nine pairs of SSR primers, there were 318 polymorphic loci, the percentage of polymorphic loci reached 95. 78%. There were significant differences between fingerprint map amplified with primer Xtxpl3 and fingerprint map amplified with primer Xtxp67, which can be used as molecular basis for identifying the eleven new strains (cultivars) of Sorghum-Sudangrass. Genetic distance (GD) among the new eleven Sorghum-Sudangrass strains ranged from 0. 4286 to 0. 7226, using the GD value of 0. 61 as threshold, the eleven new strains of Sorghum-Sudangrass can be clustered into three groups: group one: SLCN01, SLCN02, SLCN03, SLCN09, SLCN10 and Mengnong qingsi No. 3; group two: SLCN04, SLCN05, SLCN06, SLCN08; group three: SLCN07.