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食源性单核细胞增生李斯特菌快速检测研究进展

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食品安全是人类健康非常关注的问题,其中微生物污染是其主要的风险因素之一.单核细胞增生李斯特菌是一种人畜共患病的病原体,感染后主要表现为败血症、脑膜炎和单核细胞增多.该菌广泛存在于自然环境中,低温条件下仍可生长繁殖,是威胁人类健康的主要病原菌之一.传统细菌检测方法耗时长,不能满足现场检测的需求.因此开发快速、准确、高效、可靠的单核细胞增生李斯特菌检测方法是目前研究的热点.基于免疫学、分子生物学、生物传感器和噬菌体等技术的检测方法成为单核细胞增生李斯特菌检测的重要手段.该文综述各类检测技术的研究进展,阐述各个方法在致病菌检测中的原理、特点、应用现状和发展,并讨论各方法的优缺点,以期为单核细胞增生李斯特菌检测新技术的研发提供参考.
Research progress in rapid detection of food-borne Listeria monocytogenes
Bacterial contamination is one of the major problems that is considered a serious health risk factor for food safety and human health.Listeria monocytogenes(LM)is a zoonotic pathogen that can cause a variety of clinical syndromes such as reason sepsis,meningitis,and monocytosis and is widely distributed in nature.Specifically,it can grow and reproduce at low temperatures,threatening the human health of raw foods.Conventional methods of LM detection are time-consuming and cannot meet the requirements for on-site detection.Therefore,there has been an increase in demand for the development of fast,accurate,efficient,and reliable detection methods for LM in recent years.Recent rapid detection methods have been developed based on immunology,molecular biology,biosensors,and bacteriophages.Herein,we summarize the principles,characteristics,and application of various rapid detection technologies for LM,and the advantages and disadvantages of these technologies were fully discussed.Hopefully,the review can provide a reference and offer a blueprint for the research and development of new technologies for detecting LM.

food safetyListeria monocytogenesrapid detection

林琦钰、王丹庆、马沁沁

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四川师范大学生命科学学院,四川成都 610101

食品安全 单核细胞增生李斯特菌 快速检测

国家自然科学基金

31800158

2023

中国测试
中国测试技术研究院

中国测试

CSTPCD北大核心
影响因子:0.446
ISSN:1674-5124
年,卷(期):2023.49(11)
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