Determination of aflatoxins in grains using the high-throughput high efficient clean-up method and ultra-high performance liquid chromatography-tandem mass spectrometry
A simple and efficient method for determination of aflatoxins B1,B2,G1 and G2 in grains was established.By optimizing the chromatographic and mass spectrometry analysis conditions,oscillation extraction mode and dilution ratio,the optimal conditions satisfying the detection methods of aflatoxin B1,B2,G1 and G2 were determined respectively.At the same time,an high-throughput automatic presser was developed and used in combination with the flow-through clean-up column for high-throughput and efficient purification of impurities in grain.Ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was used for detection and analysis.The grinded grain sample was extracted by 84%acetonitrile solution and purified by Speedy Prep®-Myco 1 clean-up column.During UPLC-MS/MS quantification through external standard method,the purified extract was gradient eluted by ACQUITY UPLC BEH C18 column with 0.1%formic acid solution in water and acetonitrile as the mobile phase.Mass spectrometric signal collection was performed by electrospray positive ion and multiple reaction monitoring(MRM)mode.For rice,small yellow rice,wheat,yellow bean,flour,barley,black rice,peanut and corn,the limits of detection(LODs)and the limits of quantification(LOQs)of aflatoxins B1,B2,G1 and G2 were 0.06-0.12 μg/kg and 0.20-0.40 μg/kg with correlation coefficients higher than 0.9993 in their linear ranges.At spiked levels of 0.2,0.4,1,10,20 and 40 μg/kg,aflatoxins B1,B2,G1 and G2 were obtained with recoveries between 72.37%to 118.4%and relative standard deviations(RSD,n=6)between 0.64%to 14%.The recoveries and precision were good.This method is simple and stable,and is suitable for the detection of aflatoxin B1,B2,G1 and G2 in grain.
万方数据
维普
