Determination of catechins and theaflavins in tea by HPLC-MS/MS
In view of the fact that there are few methods for simultaneous determination of catechins and theaflavins in tea,a method based on high performance liquid chromatography-tandem mass spectrometry was developed for simultaneous qualitative and quantitative determination of 8 catechins and 4 theaflavins in tea.The analysis utilized Agilent Eclipse Plus C18(2.1 mm×100 mm,1.8 μm)chromatography with formic acid-acetonitrile as the mobile phase,operating at a flow rate of 0.2 mL/min for gradient elution.Multi reaction monitoring(MRM)was conducted using ESI source positive and negative ion scanning modes.The results showed that catechins and theaflavins had good linear relationships in the range of 10-10000 μg/L and 25-5000 μg/L,respectively,with correlation coefficients R＞0.99.The recovery rate is between 96.53%and 106.08%,and the relative standard deviation of precision is less than 10%.Compared with the national standard method for separately detecting catechins and theaflavins,this method improves detection efficiency and has lower detection and quantification limits,providing more accurate and comprehensive detection results.
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