HPLC-MS/MS法测定茶叶中儿茶素类、茶黄素类物质
Determination of catechins and theaflavins in tea by HPLC-MS/MS
刘培琴 1胡超琼 1邹燕 2杨潇 1陈祥贵 1冯德建 2叶善蓉 2黄玉坤1
作者信息
- 1. 西华大学食品与生物工程学院食品微生物四川省重点实验室,四川成都 610039
- 2. 中国测试技术研究院茶叶标准与检测技术四川省重点实验室,四川成都 610021
- 折叠
摘要
针对同时测定茶叶中儿茶素、茶黄素的方法较少的现状,该研究建立了同时定性和定量测定茶叶中 8种儿茶素类和 4 种茶黄素类物质的高效液相色谱-串联质谱法.研究采用Agilent Eclipse Plus C18(2.1 mm×100 mm,1.8 μm)色谱,甲酸-乙腈为流动相,流量为 0.2 mL/min进行梯度洗脱,采用ESI源正、负离子扫描模式,多反应监测(MRM).结果表明,儿茶素类和茶黄素类物质分别在 10～10000 μg/L和 25～5000 μg/L范围内线性关系良好,相关系数R＞0.99,回收率在 96.53%～106.08%之间,精密度的相对标准偏差均小于 10%.相较于分别检测儿茶素类和茶黄素类物质的国家标准方法,该方法提高了检测效率且具有更低的检出限和定量限,能够提供更加准确、全面的检测结果.
Abstract
In view of the fact that there are few methods for simultaneous determination of catechins and theaflavins in tea,a method based on high performance liquid chromatography-tandem mass spectrometry was developed for simultaneous qualitative and quantitative determination of 8 catechins and 4 theaflavins in tea.The analysis utilized Agilent Eclipse Plus C18(2.1 mm×100 mm,1.8 μm)chromatography with formic acid-acetonitrile as the mobile phase,operating at a flow rate of 0.2 mL/min for gradient elution.Multi reaction monitoring(MRM)was conducted using ESI source positive and negative ion scanning modes.The results showed that catechins and theaflavins had good linear relationships in the range of 10-10000 μg/L and 25-5000 μg/L,respectively,with correlation coefficients R＞0.99.The recovery rate is between 96.53%and 106.08%,and the relative standard deviation of precision is less than 10%.Compared with the national standard method for separately detecting catechins and theaflavins,this method improves detection efficiency and has lower detection and quantification limits,providing more accurate and comprehensive detection results.
关键词
茶叶/儿茶素/茶黄素/高效液相色谱-串联质谱Key words
tea/catechins/theaflavins/HPLC-MS/MS引用本文复制引用
基金项目
四川省重点研发项目(2020YFN0154)
茶叶标准与检测技术四川省重点实验室开放课题(STTT202103)
出版年
2024
NETL
NSTL
万方数据