超声介导基因转染增强骨髓间充质干细胞大鼠心脏归巢
Ultrasound-Mediated Gene Transfection Enhances the Homing of Bone Marrow Mesenchymal Stem Cells in Rat Heart
陈翔 1米科睿 1井昱茜 1杨灵洁 2庄丽 3王伟1
作者信息
- 1. 乌鲁木齐市友谊医院超声科 乌鲁木齐市,830049
- 2. 新疆医科大学第一附属医院心脏超声诊断科 乌鲁木齐市,830011
- 3. 新疆生产建设兵团医院超声科 乌鲁木齐市,830092
- 折叠
摘要
目的 探讨超声靶向微泡爆破(UTMD)技术介导基质细胞衍生因子-1(SDF-1)转染增强三维(3D)培养骨髓间充质干细胞(BMSCs)心脏归巢效率.方法 制备载SDF-1基因生物素化微泡(BMBs)(SDF-1/BMBs).将24只SD大鼠分组:二维(2D)培养BMSCs、3D BMSCs、3D BMSCs+BMBs、3D BMSCs+SDF-1/BMBs.UTMD介导SDF-1基因于心脏释放.14 d后各组经尾静脉注射相应BMSCs.移植后48 h检测肺脏和心脏BMSCs数量.结果 2D BMSCs组困于肺脏的数量(12.40±2.88)多于3D BMSCs;3D BMSCs+SDF-1/BMBs组心脏归巢数量最多(8.80±3.35).结论 3D BMSCs细胞直径小于2D BMSCs,通过肺循环较多;UTMD介导SDF-1基因转染可增加3D BMSCs心脏归巢数量.
Abstract
Objective To explore the efficiency of ultrasound-targeted microbubble destruction(UTMD)tech-nique mediated stromal cell derived factor-1(SDF-1)transfection in cardiac homing of three-dimensional(3D)sphere cultured bone marrow mesenchymal stem cells(BMSCs).Methods Biotinylated microbubbles(BMBs)loaded with SDF-1 gene(SDF-1/BMBs)were prepared.Then 24 Sprague Dawley(SD)rats were divided into four groups:two-dimensional(2D)cultured BMSCs;3D BMSCs;3D BMSCs+BMBs;3D BMSCs+SDF-1/BMBs.UTMD-mediated SDF-1 gene local-delivery to the heart.Fourteen days later,each group was injected with BMSCs via tail vein respec-tively.Next the BMSCs in the lung and heart were measured 48 hours after transplantation.Results The quantity of 2D BMSCs trapped inside the lungs(12.40±2.88)was significantly greater than that of 3D BMSCs.Notably,the amount of BMSCs within the 3D BMSCs+SDF-1/BMBs group exhibited the highest value(8.80±3.35)homing to the heart.Conclusions The diameter of 3D BMSCs is smaller than that of 2D BMSCs,which were trapped in the lungs less.Furthermore,SDF-1 gene transfection mediated by UTMD can significantly augment the homing capacity of 3D BMSCs.
关键词
超声靶向微泡爆破/基因/三维球体培养/骨髓间充质干细胞Key words
Ultrasound-targeted microbubble destruction/Genes/3D sphere culture/Bone marrow mesenchy-mal stem cells引用本文复制引用
出版年
2025
NETL
NSTL
万方数据