Analysis of pathogenic bacteria in the detection of periprosthetic joint infection by mNGS
Objective To explore the role of metagenomic next-generation sequencing(mNGS)in the detection of pathogenic bacteria in periprosthetic joint infection(PJI).Methods A total of 200 patients who underwent joint revision surgery in Tianjin Jinkey Medical Technology Co.,Ltd.Laboratory from January 2020 to December 2021 were selected,with including 126 knee joints and 74 hip joints.Routine bacterial culture and mNGS detection were performed on pathogenic bacteria in joint fluid or synovial tissue samples of patients.The diagnostic positivity rate,diagnostic efficacy(sensitivity,specificity,positive predictive value,negative predictive value,diagnostic consistency),and detection time differences were compared between two detection methods.Results The diagnostic positivity rate in the mNGS detection was higher than that in the conventional bacterial culture detection,and the difference was statistically significant(P＜0.05).The diagnostic sensitivity,negative predictive value and diagnostic accuracy of the mNGS detection were higher than those of the conventional bacterial culture detection,but the specificity and positive predictive value were lower than those of the conventional bacterial culture detection,and the differences were statistically significant(P＜0.05).The detection time of mNGS detection was(1.87±0.29)d,which was shorter than that of conventional bacterial culture detection with(5.17±0.59)d,and the difference was statistically significant(P＜0.05).Conclusion mNGS detection could effectively identify pathogenic bacteria in PJI patients,had high diagnostic sensitivity and diagmostic accuracy,and had a clear advantage in detection time.It had a high diagnostic efficiency in the PJI and could provide effective guidance and suggestions for the diagnosis and treatment of PJI.
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