The results analysis of UPLC-MS/MS detection of water-soluble vitamins in children's blood
Objective To explore the use of ultra-high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)technology to detect the levels of water-soluble vitamins in the blood of children and analyze the detection results.Methods A total of 930 children who underwent health examinations at the Growth and Development Outpatient Department of Liaocheng Dongchangfu District Maternal and Child Health Hospital from December 2022 to December 2023 were selected as the observation objects.The FH6000-MD UPLC-MS/M analyzer was used to detect water-soluble vitamins B1,B2,B5,B6 and B9.The detection results were statistically analyzed according to different age groups and genders.Results The differences in the contents of vitamin B2,B5,B6 and B9 in the blood of children of different age groups were statistically significant(P＜0.05).The contents of vitamin B2,B5 and B9 in the blood of girls were(9.57±4.89),(30.95±19.70)and(4.96±2.71)ng/mL,which were higher than those of boys with(8.64±4.66),(27.59±16.06)and(4.62±2.27)ng/mL,and the differences were statistically significant(P＜0.05);there were no statistically significant differences in the contents of vitamin B1 and B9 in the blood of boys and girls(P＞0.05).Conclusion By using UPLC-MS/MS technology to detect water-soluble vitamins in children's blood,it was found that there were differences in the absorption and utilization of vitamins in children of different ages and genders.With the growth of age,children's absorption and utilization abilities of water-soluble vitamins gradually decreased.Gender factors also had a certain impact on the levels of vitamins in children's bodies.Therefore,during process of children's growth and development,attention should be paid to the intake and supplementation of water-soluble vitamins,and reasonable nutrition strategies should be formulated according to age and gender characteristics,so as to promote the healthy growth of children.
Ultra-high performance liquid chromatography tandem mass spectrometry technologyWater-soluble vitaminsChildrenGrowth and developmentNutritional intervention
万方数据
维普
