C-X-C motif chemokine receptor 4 enhances the role of Toll-like receptor 2 in the formation of atherosclerotic lesions promoted by Chlamydia pneumoniae infection
Aim To explore the role of C-X-C motif chemokine receptor 4(CXCR4)in the formation of athero-sclerosis(As)induced by Chlamydia pneumoniae(C.pn)infection.Methods The As model in mice of ApoE-/-,ApoE-/-+TLR2-/-and ApoE-/-+Toll-like receptor 2(TLR2)-/-+AMD3100 induced by C.pn infection was established on the basis of high fat diet.C.pn IgG and IgM antibody levels were detected by ELISA,and C.pn specific DNA was detec-ted by PCR.Lipid deposition and As lesion area in aorta and aortic root were observed by oil red O and HE staining.Serum levels of total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDLC)and high density lipoprotein cholesterol(HDLC)were analyzed by colorimetry,and ELISA was used to measure the contents of serum inter-leukin-1β(IL-1β)and interleukin-6(IL-6).Results The ApoE-/-mice model of C.pn infection was established successfully.Compared with the control group,lipid deposition in aorta and aortic root of ApoE-/mice increased by 89.08％and 71.83％,and As lesion area increased by 34.12％after C.pn infection(all P＜0.05).Compared with the C.pn infection group,lipid deposition in aorta and aortic root reduced by 46.16％and 75.73％,and the lesion area of As decreased by 63.37％in the TLR2-/-+C.pn infection group(all P＜0.05).Compared with the TLR2-/-+C.pn infection group,lipid deposition in aorta and aortic root decreased by 26.19％and 56.94％,and the lesion area of As decreased by 22.24％in the TLR2-/-+AMD3100+C.pn infection group(all P＜0.05).Compared with the control group,serum levels of TC,TG and LDLC increased by 0.62 times,1.43 times and 1.34 times after C.pn infection,respectively,while serum contents of IL-1β and IL-6 increased by 4.10 times and 6.00 times,respectively(all P＜0.05).Compared with the C.pn infection group,serum levels of TC,TG and LDLC in the TLR2-/-+C.pn infection group decreased by 56.96％,50.41％and 66.64％,and serum contents of IL-iβ and IL-6 also decreased by 66.72％and 69.54％respectively(all P＜0.05).Compared with the TLR2-/-+C.pn infection group,serum levels of TC,TG and LDLC in the TLR2-/-+AMD3100+C.pn infection group decreased by 52.18％,58.56％and 60.61％,and serum contents of IL-1β and IL-6 and reduced by 28.84％and 43.18％,respectively(all P＜0.05).Conclusion CXCR4 enhances the roles of TLR2 in increasing the serum lipid levels and the contents of inflammatory factors,and then participates in the formation of As le-sions induced by C.pn infection.
万方数据
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