补阳还五汤加减抑制ApoE-/-小鼠动脉粥样硬化病变形成及降低血清炎症因子水平的机制

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中文摘要：[目的]基于Wnt/β连环蛋白(β-catenin)信号通路探讨补阳还五汤加减通过调控血管平滑肌细胞的表型转化及血清炎症因子水平,发挥抗动脉粥样硬化(As)的作用.[方法]将50只ApoE-/-小鼠随机分为模型组、补阳还五汤加减(低、中、高剂量)组及阿托伐他汀组,每组10只;10只C57BT/6J小鼠设为对照组,给予普通饲料喂养,其余5组小鼠给予高脂饲料喂养8周进行As模型复制,第9周开始灌胃,连续灌胃4周,12周后分离小鼠主动脉根部,制备石蜡切片,油红O染色观察主动脉窦脂质含量;免疫组织化学法检测骨桥蛋白(OPN)表达水平;ELISA法检测小鼠血清中白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)、白细胞介素1 β(IL-1β)、单核细胞趋化蛋白1(MCP-1)的水平;Western blot检测小鼠主动脉中Wnt1、β-catenin及c-myc蛋白的表达;RT-PCR法检测小鼠主动脉中Wnt1、β-catenin基因的表达.[结果]补阳还五汤加减(低、中、高剂量)能够降低As模型小鼠主动脉窦脂质含量及OPN表达;降低小鼠血清IL-6、TNF-α、IL-1β、MCP-1水平;并可下调小鼠主动脉中Wnt1、β-catenin蛋白及基因的表达.[结论]补阳还五汤加减通过调控炎症反应发挥抗As的作用,部分机制可能与抑制Wnt/β-catenin信号通路活化有关.

外文标题：Study on the mechanism of Buyang Huanwu decoction in inhibiting atherosclerotic le-sion formation and reducing serum inflammatory factors in ApoE-/-mice

外文摘要：Aim To explore the effect of Buyang Huanwu decoction on anti-atherosclerosis by regulating the phe-notypic transformation of vascular smooth muscle cells and serum inflammatory response based on Wnt/β-catenin signaling pathway.Methods 50 ApoE-/-mice were randomly divided into model group,Buyang Huanwu decoction modified(low,medium and high dose)groups,and atorvastatin group,with 10 mice in each group;10 C57BL/6J mice with the same genetic background were set as the control group.The mice of control group were fed with regular forages,while the mice of other five groups were fed with high-fat forages for 8 weeks for model replication.From the 9th week,they were continuously gavaged for 4 weeks.After 12 weeks,the mouse aortic roots were isolated and paraffin sections were pre-pared.Oil red O staining was used to observe the lipid content of aortic sinus.Immunohistochemical method was used to detect the expression level of bone bridge protein(OPN).Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of interleukin-6(IL-6),tumor necrosis factor-α(TNF-ot),interleukin-1β(IL-1β)and monocyte che-moattractant protein-1(MCP-1)in serum.Western blot was used to detect the protein expressions of Wnt1,β-catenin and c-myc in mice aorta.RT-PCR was used to detect the expressions of Wnt1 and β-catenin gene.Results Buyang Huanwu decoction modified(low,medium and high dose)could reduce the lipid content and OPN expression in the aortic sinus of As model mice,reduce serum levels of IL-6 and TNF-α,IL-1β and MCP-1 in mice,and downregulate the expres-sion of Wnt1 and β-catenin protein and gene.Conclusion Buyang Huanwu decoction modified exerts an anti-athero-sclerosis effect by regulating inflammatory response,and some of the mechanisms may be related to inhibiting the activation of the Wnt/β-catenin signaling pathway.

外文关键词：

atherosclerosisBuyang Huanwu decoction modifiedphenotypic transformation of vascular smooth muscle cellsinflammatory responseWnt/β-catenin signaling pathway

作者：

范增光、袁野

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作者单位：

江西中医药大学附属医院,江西省南昌市 330006

关键词：

动脉粥样硬化补阳还五汤加减平滑肌细胞表型转化炎症反应 Wnt/β-catenin信号通路

基金：

江西省卫生健康委科技计划项目江西省自然科学基金项目江西省中医药管理局科技计划项目

项目编号：

20221076020224BAB2160972022A089

出版年：

2024

DOI：

10.20039/j.cnki.1007-3949.2024.04.002

中国动脉硬化杂志

中国病理生理学会南华大学

中国动脉硬化杂志

CSTPCD

影响因子：1.086

ISSN：1007-3949

年,卷(期)：2024.32(4)

参考文献量18