首页|葛花提取物通过激活PPARγ上调ABCA1的表达而抑制THP-1源性泡沫细胞形成

葛花提取物通过激活PPARγ上调ABCA1的表达而抑制THP-1源性泡沫细胞形成

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[目的]探寻葛花提取物(PFE)对巨噬细胞源性泡沫细胞脂质蓄积的影响.[方法]通过MTT法筛选PFE对THP-1源性泡沫细胞的作用浓度,采用油红0染色和胆固醇检测试剂盒检测细胞内脂质蓄积情况,采用胆固醇流出试剂盒检测细胞的胆固醇流出水平,使用RT-qPCR和Western blot检测mRNA和蛋白表达.[结果]PFE显著降低THP-1源性泡沫细胞内的脂质蓄积.PFE不影响CD36、清道夫受体A Ⅰ(SR-AⅠ)、固醇调节元件结合蛋白2(SREBP2)及3-羟基-3-甲基戊二酰辅酶A还原酶(HMGCR)的mRNA表达,但能上调ATP结合盒转运体A1(ABCA1)的mRNA和蛋白表达水平(P＜0.05),促进巨噬细胞源性泡沫细胞胆固醇流出(P＜0.01).PFE可以激活过氧化物酶体增殖物激活受体γ(PPARγ)的活性(P＜0.01)及上调其mRNA和蛋白表达水平(P＜0.05).与PFE对照组相比,加入GW9662处理后PPARγ和ABCA1的蛋白表达水平均下降(P＜0.01),胆固醇流出水平降低(P＜0.01).[结论]PFE可显著改善THP-1源性泡沫细胞内的脂质蓄积,通过PPARγ上调ABCA1表达及其介导的胆固醇流出抑制泡沫细胞形成.
Pueraria Lobata Flowers Extract inhibits THP-1-derived foam cell formation by acti-vating PPARγ to upregulate the expression of ABCA1
Aim To explore the effect of Pueraria Lobata Flowers Extract(PFE)on lipid accumulation in mac-rophage-derived foam cells.Methods The concentration of PFE in THP-1-derived foam cells was screened by MTT,intracellular lipid accumulation was detected by oil red O staining and cholesterol detection kit,intracellular cholesterol ef-flux levels were detected by cholesterol efflux assay kit,RT-qPCR and Western blot were used to analyze mRNA and pro-tein expression.Results PFE significantly reduced lipid accumulation in THP-1-derived foam cells.PFE did not affect the mRNA expression of CD36,scavenger receptor-A Ⅰ(SR-A Ⅰ),sterol regulatory element-binding protein 2(SREBP2),3-hydroxy-3-methylglutaryl-CoA reductase(HMGCR),but it could upregulate the mRNA and protein expres-sion levels of ATP-binding cassette transporter A1(ABCA1)(P＜0.05),and promote the intracellular cholesterol efflux of macrophage-derived foam cells(P＜0.01).PFE could activate the activity of peroxisome proliferator-activated receptor y(PPARγ)(P＜0.01)and upregulate the mRNA and protein expression levels of PPARγ(P＜0.05).Compared with the PFE control group,the expression of PPARγ and ABCA1 proteins decreased and cholesterol efflux decreased after GW9662 treatment(all P＜0.01).Conclusion PFE could significantly prevent the lipid accumulation in THP-1-derived foam cells and inhibit the formation of foam cells by upregulating ABCA1 expression and cholesterol efflux mediated by PPARγ.

Pueraria Lobata Flowers Extractfoam cellATP-binding cassette transporter A1peroxisome proliferator-activated receptor γ

朱容蓉、陈梦娇、赵真旺、刘嘉怡、吴剑锋、王宇菲、张敏

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南华大学心血管疾病研究所 动脉硬化学湖南省重点实验室 湖南省动脉硬化性疾病国际科技创新合作基地南华大学生物信息与医学大数据教研室,湖南省衡阳市 421001

湖北文明学院医学部基础医学院,湖北省襄阳市 441053

湖南医药学院公共卫生与检验医学院,湖南省怀化市 418000

南华大学附属第二医院心血管内科,湖南省衡阳市 421001

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葛花提取物 泡沫细胞 ATP结合盒转运体A1 过氧化物酶体增殖物激活受体γ

湖南省教育厅科学研究项目湖北省自然科学基金青年项目湖北省自然科学基金青年项目大学生创新创业项目

19B4902023AFB0432021JJ40485S202112214008

2024

10.20039/j.cnki.1007-3949.2024.05.004

中国动脉硬化杂志
中国病理生理学会 南华大学

中国动脉硬化杂志

CSTPCD
影响因子：1.086
ISSN：1007-3949
年,卷(期)：2024.32(5)