Objective To explore the effect of long non-coding RNA nuclear enriched transcriptome 1(lncRNA NEAT1)on the migration and invasion of laryngeal squamous cell carcinoma(LSCC)cells,and further explore the role of miR-429/zinc finger E-box binding homeobox 1(ZEB1)axis in this process.Methods Lentivirus transfection established LSCC cells with stable knockdown lncRNA NEAT1,and lncRNA NEAT1 expression was detected by real-time quantitative polymerase chain reaction(RT-qPCR)to verify transfection efficiency.Cell migration and invasion ability were detected by transwell assay.The expressions of epithelial-mesenchymal transition(EMT)related proteins N-cadherin,Vimentin,Slug and Snail were detected by Western blot.The potential binding sites between miR-429 and lncRNA NEAT1 and ZEB1 mRNA were analyzed by bioinformatics,and the binding of miR-429 to lncRNA NEAT1 and miR-429 to ZEB1 mRNA were verified by dual luciferase reporter gene assay.LSCC cells with knockdown lncRNA NEAT1 were transfected with miR-429 inhibitors,and ZEB1 expression was detected by Western blot.The effect of knockdown ZEB1 on LSCC cell migration,invasion and EMT of miR-429 knockdown lncRNA NEAT1 was further examined.Results Knocking down lncRNA NEAT1 reduced lncRNA NEAT1 expression in LSCC cells,inhibited cell migration and invasion,and down-regulated the expression of N-cadherin,Vimentin,Slug,and Snail.There were potential binding sites between miR-429 and lncRNA NEAT1 and ZEB1 mRNA,and miR-429 bound to lncRNA NEAT1 and miR-429 to ZEB1 mRNA.Inhibition of miR-429 reversed the inhibitory effect of knocking down lncRNA NEAT1 on ZEB1 expression in LSCC cells.In addition,knockdown of ZEB1 reversed the inhibitory effect of miR-429 on the migration and invasion of LSCC cells with knockdown lncRNA NEAT1,as well as the upregulation of EMT related proteins N-cadherin,Vimentin,Slug,and Snail expression.Conclusion LncRNA NEAT1 promotes migration and invasion of LSCC cells,and the mechanism may be related to the upregulation of ZEB1 expression by sponging miR-429 and promoting EMT in LSCC cells.
Laryngeal squamous cell carcinomaLncRNA NEAT1MiR-429/ZEB1 axisCell migration and invasionEpithelial-mesenchymal transformation
维普
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