Methylation level of CNR1 in peripheral blood of children with autism spectrum disorder
Objective To explore the relationship between the methylation level of CNR1 and autism spectrum disorder(ASD),in order to provide a theoretical basis for the etiology of ASD.Methods A case-control study was conducted,recruiting 30 children with ASD from the Child Development and Behavior Research Center of Harbin Medical University and a rehabilitation facility,and 30 matched typically developed children from June 2017 to December 2018.The methylation levels of CNR1 in peripheral blood were measured by the Agena MassArray® Mass Spectrometry System.A univariate conditional Logistic regression model was used to analyze the potential association between the methylation level of CNR1 and the risk of ASD with adjustment for age,BMI,body fat percentage and body fat.The correlations between the methylation level of CNR1 and the score of Social Responsiveness Scale(SRS)were evaluated by Pearson/Spearman correlation analysis.Results The methylation levels of the average methylation(t=2.224),CpG_3.4(Z=2.187),CpG-9.10.11(t=2.308),and CpG_28.29(t=2.943)of the CNR1 promoter region in ASD children were significantly higher than controls(P＜0.05).The methylation levels of the average methylation(OR=1.117,95％CI:1.003-1.245),CpG_9.10.11(OR=1.072,95％CI:1.006-1.142),and CpG_28.29(OR=1.078,95％CI:1.018-1.141)of the CNR1 promoter region were positively correlated with the risk of ASD(P＜O.05).The methylation level of CpG_28.29 in ASD children was positively correlated with the scores of social motivation in SRS(r=0.421,P＜0.05).Conclusions The methylation levels of CNR1 in peripheral blood are abnormal in ASD children and might be correlated with the risk of ASD and social function.The underlying mechanism needs to be further explored.
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