Effects of different feeding methods on intestinal microbiota in infants with cow s milk protein allergy
Objective To explore the effects of various feeding methods on the gut microbiota of infants with cow's milk protein allergy(CMPA),in order to provide theoretical guidance for the rational selection of intestinal microecological treatment for infants with CMPA.Methods Fecal samples of 24 infants with CMPA and 26 healthy infants were collected from September 2020 to October 2021 in the Affiliated Hospital of Zunyi Medical University.Bacterial DNA was extracted from these samples,followed by PCR amplification of the V3-V4 region of the 16S rDNA.High-throughput sequencing was then conducted for bacterial classification.The structure and diversity of intestinal flora of CMPA infants with different feeding methods were analyzed from four aspects:beta diversity analysis,alpha diversity analysis,species composition analysis and species difference analysis.Results Infants with CMPA who were exclusively breastfed exhibited greater gut microbiota diversity compared to healthy breastfed infants(P＜0.05).Conversely,there was no significant difference in intestinal microflora diversity between mixed-fed CMPA infants and mixed-fed healthy infants,formula-fed CMPA infants and formula-fed healthy infants(P＞0.05).Notable distinctions included enriched populations of Bifidobacterium and Escherichia-Shigella in breastfed CMPA infants,Streptococcus and Rothia in mixed-fed CMPA infants,and unclassified Enterobacteriaceae and Citrobacter in formula-fed CMPA infants.Conclusion Intestinal flora disorder exists in infants with different feeding patterns of CMPA,which may provide some theoretical basis for the study of pathogenesis and microecological intervention of CMPA with different feeding patterns.
cow's milk protein allergyintestinal microbiotafeeding patternhigh-throughput sequencinginfants
NETL
NSTL
万方数据
