不同喂养方式对牛奶蛋白过敏患儿肠道菌群的影响
Effects of different feeding methods on intestinal microbiota in infants with cow s milk protein allergy
罗光月 1陆韦 2徐德勇 2邱政飞 2李宗龙2
作者信息
- 1. 黔南州人民医院儿科,贵州都匀 558000
- 2. 遵义医科大学附属医院儿科/贵州省儿童医院
- 折叠
摘要
目的 探讨不同喂养方式对牛奶蛋白过敏(CMPA)患儿肠道菌群的影响,以期为临床合理选用肠道微生态治疗CMPA患儿提供理论指导.方法 收集2020年9月—2021年10月于遵义医科大学附属医院就诊CMPA患儿(24例)和健康婴儿(26例)的粪便,提取粪便细菌DNA,对16S rDNA V3-V4区进行PCR扩增,并在高通量测序后进行细菌学分类,从Beta多样性、Alpha多样性、物种组成分析、物种差异分析4个方面,分析不同喂养方式CMPA患儿肠道菌群的多样性及结构差异.结果 母乳喂养的CMPA患儿肠道菌群多样性高于母乳喂养的健康婴儿(P＜0.05),混合喂养的CM-PA患儿与混合喂养的健康婴儿、人工喂养的CMPA患儿与人工喂养的健康婴儿相比,肠道菌群多样性差异无统计学意义(P＞0.05),显著差异菌为:母乳喂养的CMPA患儿双歧杆菌属、大肠杆菌-志贺氏菌属显著富集,混合喂养的CMPA患儿链球菌属、罗氏菌属显著富集,人工喂养的CMPA患儿未分类的肠杆菌属、枸橼酸杆菌属显著富集.结论 不同喂养方式CMPA婴儿存在肠道菌群紊乱,该结果可能为不同喂养方式CMPA的发病机制研究及其微生态干预提供部分理论依据.
Abstract
Objective To explore the effects of various feeding methods on the gut microbiota of infants with cow's milk protein allergy(CMPA),in order to provide theoretical guidance for the rational selection of intestinal microecological treatment for infants with CMPA.Methods Fecal samples of 24 infants with CMPA and 26 healthy infants were collected from September 2020 to October 2021 in the Affiliated Hospital of Zunyi Medical University.Bacterial DNA was extracted from these samples,followed by PCR amplification of the V3-V4 region of the 16S rDNA.High-throughput sequencing was then conducted for bacterial classification.The structure and diversity of intestinal flora of CMPA infants with different feeding methods were analyzed from four aspects:beta diversity analysis,alpha diversity analysis,species composition analysis and species difference analysis.Results Infants with CMPA who were exclusively breastfed exhibited greater gut microbiota diversity compared to healthy breastfed infants(P＜0.05).Conversely,there was no significant difference in intestinal microflora diversity between mixed-fed CMPA infants and mixed-fed healthy infants,formula-fed CMPA infants and formula-fed healthy infants(P＞0.05).Notable distinctions included enriched populations of Bifidobacterium and Escherichia-Shigella in breastfed CMPA infants,Streptococcus and Rothia in mixed-fed CMPA infants,and unclassified Enterobacteriaceae and Citrobacter in formula-fed CMPA infants.Conclusion Intestinal flora disorder exists in infants with different feeding patterns of CMPA,which may provide some theoretical basis for the study of pathogenesis and microecological intervention of CMPA with different feeding patterns.
关键词
牛奶蛋白过敏/肠道菌群/喂养方式/高通量测序/婴儿Key words
cow's milk protein allergy/intestinal microbiota/feeding pattern/high-throughput sequencing/infants引用本文复制引用
基金项目
遵义医学院附属医院博士科研启动基金合同书(院字201401号)
遵义市科技局联合基金(遵市科合HZ字2020244号)
遵义市科技局联合基金(遵市科合HZ字2022363号)
贵州省科技计划项目(黔科合基础-ZK[2024]312号)
出版年
2024
NETL
NSTL
万方数据