Role and significance of EMP1 in cisplatin resistance of epithelial ovarian cancer
Objective To investigate the role and clinical significance of epithelial membrane protein 1(EMP1)in cisplatin resistance in epithelial ovarian cancer(EOC).Methods A total of 56 paraffin samples from EOC recurrence reoperation were collected,and the expression of EMP1 protein in EOC tissues was detected by immunohistochemistry,and the expression of protein was described by integrated optical density(IOD).Real-time PCR(qRT-PCR)and Western blot were used to detect the expression of EMP1 mRNA and protein in SKOV3 and SKOV3/DDP cell lines.Cox regression analysis was used to assess its prognostic impact.Results Immunohistochemical results showed that the IOD value of EMP1 protein in cisplatin-sensitive group was 9.45±3.42,and the value was 17.70±8.10 in the cisplatin-resistant group,which was statistically significant(P＜0.001).qRT-PCR results showed that the expression of EMP1 mRNA in SKOV3/DDP cells was significantly higher than in SKOV3 cells(P＜0.001).Western blot results showed that the expression of EMP1 protein in SKOV3/DDP cells was higher than SKOV3 cells(P=0.002).The ROC curve determined the cut-off values of EMP1 expression at high and low levels,the cut-off value of IOD was 11.37,the area under the ROC curve was 8.821,the sensitivity was 80.65％,and the specificity was 80％.All patients have been followed to the end of life,and the median overall survival time(OS)in the EMP1 high-expression group was 14.0 months(range 8.0～33.0 months),while the low expression group was 23.0 months(range 9.0～55.0 months).Cox regression analysis showed that EMP1 expression level was significantly correlated with OS(HR=5.936,P＜0.001).Conclusions EMP1 can be used as a marker of cisplatin resistance and an independent risk factor for the prognosis of EOC.
epithelial membrane protein 1epithelial ovarian cancercisplatin resistancemarkers
万方数据
维普
