Non targeted metabonomic analysis of pregnant women and infants with diabetes mellitus and study on the correlation between their offspring based on liquid mass technology
Objective To conduct the maternal and fetal non targeted metabonomics analysis of gestational diabetes(GDM)based on liquid chromatography mass spectrometry(LC-MS),and to analyze the relationships between other indicators and offspring to guide the clinical data of GDM.Methods From January 2022 to November 2022,GDM and healthy pregnancy who underwent elective cesarean section at full term in the our hospital were selected and recorded as GDM group(n=30)and control group(n=30)respectively.Using LC-MS method,peripheral blood and umbilical cord blood were collected from both groups for non targeted metabolomics analysis,and the birth weight,blood glucose,blood gas analysis,1-minute Apgar scores and bilirubin at different moments of the two groups were compared.Results ® The main metabolites in maternal blood and cord blood in GDM group and control group increased metabolites in maternal blood included lysophosphatidylcholine 18:2 metabolite,polyethylene,etc,and the decreased metabolites included 3-amino-lH-pyrazolo[4,3-c]pyridine-4,6-diol,N-diethyldodecylamine,etc.Increased metabolites in cord blood included glycine cholic acid,octadecanoic acid,etc,and the decreased metabolites included 1,3-dimethyluric acid,3-hydroxy-2-octylglutaric acid,etc.® According to partial least squares discriminant analysis(PLS-DA),the model was stable and reliable.® The newborn birth weight,birth blood sugar,24-hour bilirubin,48-hour bilirubin in GDM group were(3 165.82±210.88)g,(4.56±0.36)mmol/L,(93.08±34.31)μmol/L and(154.80±39.89)μmol/L,which were(2 763.24±212.57)g,(4.06±0.40)mmol/L,(73.40±24.27)μmol/L and(132.83±29.57)μmol/L in the healthy control group,and those in the GDM group were higher than those in the healthy control group(P＜0.05).Conclusions The increased metabolites in GDM maternal and cord blood may affect fetal growth and development,and it is guessed that they are speculated to be related to newborn birth weight,birth blood sugar,24-hour bilirubin,and 48-hour bilirubin.
万方数据
维普
