重度子痫前期孕妇胎盘组织中Treg细胞及其相关因子的表达意义研究
Expression of Treg cells and related factors in placental tissue of pregnant women with severe pre-eclampsia
刘涛 1李兰1
作者信息
- 1. 贵州中医药大学第一附属医院重症医学科,贵阳 550001
- 折叠
摘要
目的 探讨Treg细胞及其相关因子在重度子痫前期(SPE)孕妇胎盘组织中的表达和意义.方法 纳入贵州中医药大学第一附属医院重症医学科2020年1月至2022年1月收治的74例SPE患者(SPE组)和本院同期75例正常足月生产的产妇(对照组)作为研究对象.收集研究对象基线资料及实验室检查数据,检测研究对象外周血CD4+CD25+Treg细胞的表达水平,荧光定量聚合酶链反应(PCR)检测外周血Treg细胞转录因子Foxp3mRNA、转化生长因子β1(TGF-β1)表达水平,采用蛋白印迹法(Western blot)检测胎盘组织中Foxp3、TGF-β1蛋白表达水平.结果 SPE组患者血清CD4+CD25+Treg占比低于对照组;SPE组患者血清IL-10、TGF-β1水平均高于对照组(P＜0.05),HLA-G水平低于对照组(P＜0.05);SPE组患者血清、胎盘组织中Foxp3 mRNA表达水平低于对照组(P＜0.05),血清、胎盘组织中TGF-β1 mRNA表达水平高于对照组(P＜0.05);SPE组胎盘组织中Foxp3蛋白表达量低于对照组,TGF-β蛋白表达量高于对照组;SPE患者外周血TGF-β1水平与胎盘组织TGF-β1蛋白水平呈正相关关系(r=0.645,P＜0.05).结论 SPE患者外周血和胎盘组织中Treg细胞相关因子(Foxp3、TGF-β1)的表达均发生改变.
Abstract
Objective To investigate the expression and significance of Treg cells and related factors in placental tissues of pregnant women with severe pre-eclampsia(SPE).Methods A total of 74 patients with SPE(SPE group)and 75 women with Full-term production(control group)admitted between January 2020 and January 2022 were included as study subjects.Baseline data and laboratory examination data were collected from the study subjects,and the expression levels of CD4+CD25+Treg cells in peripheral blood were detected,and the expression levels of transcription factor Foxp3 mRNA and transforming growth factor β1(TGF-β1)in peripheral blood Treg cells were detected by fluorescence quantitative polymerase chain reaction(PCR),and protein blotting(Western blot)was used to the expression levels of Foxp3 and TGF-β1 protein in placental tissues were detected.Results The percentage of serum CD4+CD25+Treg was lower in the SPE group than in the control group;the serum IL-10 and TGF-β1 levels were higher in the SPE group than in the control group(P＜0.05),and the HLA-G levels were lower than in the control group(P＜0.05);the expression levels of Foxp3 mRNA in serum and placental tissues were lower in the SPE group than in the control group(P＜0.05).The expression levels of TGF-β1 mRNA in serum and placental tissues were higher in the SPE group than in the control group(P＜0.05);the expression level of Foxp3 protein in placental tissue of SPE group was lower than that of control group,and TGF-β protein expression level was higher than that of the control group;There was a positive correlation between TGF-β1 level in peripheral blood of SPE patients and TGF-β1 protein level in placental tissue(r=0.645,P＜0.05).Conclusion The expression of Treg cell-related factors(Foxp3,TGF-β1)in peripheral blood and placental tissues of SPE patients was changed.
关键词
重度子痫前期/胎盘组织/Treg细胞/Foxp3/转化生长因子βKey words
severe pre-eclampsia/placental tissue/Treg cells/Foxp3/transforming growth factor β引用本文复制引用
基金项目
贵州省卫生健康委科学技术基金(Gzwkj2022-293)
出版年
2024
NETL
NSTL
万方数据