Clinical application of point-of-care testing for rapid detection of human papillomavirus in high-risk population
Objective To investigate the clinical applicability of human papillomavirus(HPV)point-of-care testing(POCT)in screening cervical intraepithelial lesions in high-risk populations.Methods In 499 patients with colposcopic referral indication,HPV POCT method and Sanger sequencing were used for HPV detection,and the consistency of HPV POCT rapid detection method was compared to evaluate the accuracy of HPV detection.Combined with the pathological results of patients,the clinical prediction performance of cervical intraepithelial lesions in high-risk population was analyzed.Results Among 499 patients,the overall HPV infection rate was 82.57％(412/499).The overall coincidence rate between HPV POCT method and Sanger sequencing technology was 90.49％(1 094/1 209).Taking Sanger sequencing detection results as a reference,the sensitivity,specificity,accuracy,positive predictive value and negative predictive value of HPV POCT method were higher than 90.00％,and the Kappa consistency coefficient was greater than 0.90(P＜0.001).The sensitivity and specificity of HPV POCT for screening of cervical intraepithelial neoplasia(CIN)2+and CIN 3 is comparable to that of Sanger sequencing.Conclusions HPV POCT method demonstrated high consistency with Sanger sequencing in terms of HPV detection and CIN 2+prediction.
human papillomavirusinstant detectioncervical intraepithelial neoplasiahigh risk population
NETL
NSTL
万方数据
