妇宁阴道膨胀栓对人乳头瘤病毒阳性宫颈癌SiHa细胞的抑制作用
The inhibitory effect of Funing vaginal suppository on human papillomavirus-positive cervical cancer SiHa cells
申卓未 1李亚芹 1罗红学 1高蕾 1王甲琪 1贾元元 1张悦 1吴晓彤 1王青 1郑诗雯 1孙秀丽1
作者信息
- 1. 北京大学人民医院妇产科,北京 100044
- 折叠
摘要
目的 探究妇宁阴道膨胀栓对宫颈癌细胞系(SiHa)的抑制、增殖、迁移和凋亡的影响.方法 取对数生长期的人乳头瘤病毒(HPV)16阳性宫颈癌细胞系(SiHa),随机分为对照组和药物组.采用10％胎牛血清的DMEM培养基常规培养作为对照组,采用10％胎牛血清DMEM培养基和妇宁阴道膨胀栓溶液配制药物组,药物浓度分别为0、0.01、0.1、1、10、30和100mg/mL.采用不同浓度的药物溶液处理SiHa细胞48 h和120 h,应用细胞增殖和毒性检测(CCK-8法)计算药物最适作用浓度;采用CCK-8法和免疫荧光染色法检测药物对SiHa细胞增殖能力的影响;采用细胞迁移实验(Transwell法)评估药物对SiHa细胞迁移能力的影响;使用流式细胞术检测细胞凋亡.结果 ①与对照组相比,0.01 g/mL及其以上药物浓度下对SiHa细胞作用48 h和120 h后,450 nm波长下每孔细胞吸光度值(OD值)明显降低(P＜0.05);②0.01 g/mL药物浓度对SiHa细胞作用4 d,药物组与对照组免疫荧光ki-67的荧光面积和ki-67/DAPI阳性率均存在显著差异(P=0.048;P=0.023);③0.01 g/mL药物浓度对SiHa细胞作用4 d,药物组与对照组迁移SiHa细胞的数量均存在显著差异(P=0.006);④0.01 g/mL药物浓度对SiHa细胞作用4 d后细胞凋亡数明显增加(P＜0.001).结论 妇宁阴道膨胀栓对SiHa细胞的增殖和迁移存在显著的抑制效果,并且促进SiHa细胞凋亡,这可能为预防HPV16感染患者进一步发展为宫颈癌提供新的诊疗思路.
Abstract
Objective To explore the effects of Funing vaginal dilation supposit on the inhibition,proliferation,migration and apoptosis of cervical cancer cell line(SiHa).Methods Human papillomavirus infection(HPV)16 positive cervical cancer cell line(SiHa)in logarithmic growth phase was randomly divided into control group and drug group.The DMEM medium with 10％fetal bovine serum was used as the control group,and the DMEM medium with 10％fetal bovine serum and Funing vaginal suppository solution were used to prepare the drug group with concentrations of 0,0.01,0.1,1,10,30 and 100 mg/mL,respectively.SiHa cells were treated with different concentrations of drug solutions for 48 h and 120 h,and the optimal drug concentration was calculated by cell proliferation and toxicity detection(CCK-8 method).CCK-8 method and immunofluorescence staining were used to detect the effect of drugs on the proliferation ability of SiHa cells.Cell migration assay(Transwell)was used to evaluate the effect of drugs on the migration ability of SiHa cells;Apoptosis was detected using flow cytometry.Results ① Compared to the control group,a significant reduction in the OD value of SiHa cells was observed after treatment with 0.01 g/mL or higher drug concentrations for 48 h and 120 h(P＜0.05).(2)After 4 days of treatment with 0.01 g/mL drug concentration,there were significant differences in the fluorescent area of immunofluorescence Ki-67 and the Ki-67/DAPI ratio between the drug-treated group and the control group(P=0.048;P=0.023).③ A significant difference was observed in the number of migrated SiHa cells between the drug-treated group and the control group after 4 days of treatment with 0.01g/mL drug concentration(P=0.006).④ There was a significant increase in the number of apoptotic cells after 4 days of treatment with 0.01g/mL drug concentration(P＜0.001).Conclusions Funing vaginal suppository has a significant inhibitory effect on the proliferation and migration of cervical cancer cells(SiHa),and promote apoptosis of SiHa cells.This may provide new diagnostic and therapeutic ideas for preventing patients with HPV 16 positive infection from further developing cervical cancer.
关键词
宫颈癌/人乳头瘤病毒感染/苦参碱/中药治疗Key words
cervical cancer/human papillomavirus infection/matrine/Chinese herbal medicine treatment引用本文复制引用
出版年
2024
NETL
NSTL
万方数据