This paper takes the global patent data of nano-biocatalytic technology on PatSnap platform as the research object.It analyzes the global patent application trend,application region,innovation subject,technology composition,legal status and technology route of nano-biocatalytic technology by using the patent metrology analysis method,so as to reveal the development trend of the latest nano-biocatalytic technology as well as the hotspots of the research,and to put forward recommendations for the development of the technology in this field as well as for the layout of the patents.It also puts forward suggestions on the development of technology and patent layout in this field for China in the direction of R&D and application of nano-biocatalytic technology.