Effects of occupational radiation exposure on peripheral blood lymphocyte count and cell cycle in logging workers
Objective To investigate the effects of prolonged low-dose neutron-γ radiation on peripheral blood lympho-cytes of logging workers.Methods The health information of workers in a logging company was collected by on-site blood sample collection and questionnaire survey.Individual doses of y and neutron radiation were recorded using LiF ele-ments and CR-39,respectively.Lymphocyte count in peripheral blood was measured by blood cytometer.Cell cycle and cyc-lins were detected by flow cytometry.Results The annual dose of some logging workers exceeded 5 mSv.Lymphocyte counts showed a difference of 15％between the group exposed to the lowest annual dose of 0-1 mSv(mean:2.45 × 109/L)and the group exposed to the highest annual dose of 5-25 mSv(mean:2.08 × 109/L).In comparison to pre-shift workers,log-ging workers exhibited a G1-phase arrest in the lymphocyte cycle,along with increased expression of cyclins p21 and CDK2.Conclusion Prolonged exposure to low-dose neutron-γ radiation leads to reduced lymphocyte counts as well as changes in lymphocyte cycle and cyclin expression.
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