职业照射对测井工人外周血淋巴细胞数量和细胞周期的影响
Effects of occupational radiation exposure on peripheral blood lymphocyte count and cell cycle in logging workers
李卫国 1高刚 2潘艳 2李海亮 3阮健磊 2朴春南 2傅惊虹 3刘建香2
作者信息
- 1. 中国疾病预防控制中心辐射防护与核安全医学所,辐射防护与核应急中国疾病预防控制中心重点实验室,北京 100088;山东第一医科大学(山东省医学科学院)预防医学科学学院(放射医学研究所),山东济南 250117
- 2. 中国疾病预防控制中心辐射防护与核安全医学所,辐射防护与核应急中国疾病预防控制中心重点实验室,北京 100088
- 3. 山东第一医科大学(山东省医学科学院)预防医学科学学院(放射医学研究所),山东济南 250117
- 折叠
摘要
目的 分析长期低剂量中子-γ辐射对测井工人外周血淋巴细胞的影响.方法 通过现场采集血样和问卷调查获取测井公司工人健康信息,采用LiF圆片和CR-39监测测井工人的γ和中子剂量,应用血细胞计数仪检测外周血淋巴细胞计数,应用流式细胞术检测细胞周期及周期相关蛋白的变化情况.结果 部分测井工人年受照剂量超过5 mSv.淋巴细胞计数在最小年剂量0～1 mSv组均值为2.45 × 109/L,在最大年剂量5～25 mSv组均值为2.08 × 109/L,差值比达到15％.与岗前工人相比,测井工人淋巴细胞周期发生G1期阻滞,周期蛋白p21、CDK2表达增加.结论 长期低剂量中子-γ辐射可引起人淋巴细胞计数下降,也可引起淋巴细胞周期和周期蛋白表达改变.
Abstract
Objective To investigate the effects of prolonged low-dose neutron-γ radiation on peripheral blood lympho-cytes of logging workers.Methods The health information of workers in a logging company was collected by on-site blood sample collection and questionnaire survey.Individual doses of y and neutron radiation were recorded using LiF ele-ments and CR-39,respectively.Lymphocyte count in peripheral blood was measured by blood cytometer.Cell cycle and cyc-lins were detected by flow cytometry.Results The annual dose of some logging workers exceeded 5 mSv.Lymphocyte counts showed a difference of 15％between the group exposed to the lowest annual dose of 0-1 mSv(mean:2.45 × 109/L)and the group exposed to the highest annual dose of 5-25 mSv(mean:2.08 × 109/L).In comparison to pre-shift workers,log-ging workers exhibited a G1-phase arrest in the lymphocyte cycle,along with increased expression of cyclins p21 and CDK2.Conclusion Prolonged exposure to low-dose neutron-γ radiation leads to reduced lymphocyte counts as well as changes in lymphocyte cycle and cyclin expression.
关键词
测井工人/中子-γ辐射/淋巴细胞计数/细胞周期/周期蛋白Key words
Logging worker/Neutron-γ radiation/Lymphocyte count/Cell cycle/Cyclin引用本文复制引用
基金项目
山东省医药卫生科技发展计划(202202080554)
山东省医科院放射医学研究所发展基金(2023-1)
山东省大学生科技创新项目(第一批)(2023)()
出版年
2024
NETL
NSTL
万方数据