Differential mRNA expression in fractional low-dose radiation-induced senescence of HBE cells
Objective To explore the differentially expressed mRNAs and related biological processes and pathways in fractional low-dose ionizing radiation(LDIR)-induced senescence of normal human bronchial epithelial(HBE)cells by high-throughput mRNA sequencing and bioinformatics techniques.Methods Senescence-associated β-galactosidase staining and senescence-associated secretion phenotype gene mRNA and protein expression levels were measured at 24 and 48 h after irradiating HBE cells 7 times at doses of 0,50,100,and 200 mGy,respectively.The differentially expressed genes were screened by high-throughput sequencing for Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analyses.Results The senescence-positive area of fractional low-dose irradiated HBE cells increased in a dose-dependent manner(P＜0.05).The mRNA levels and protein expression of transforming growth factor-β1(TGF-β1)and matrix metallo-proteinase-9(MMP-9)genes were increased in the 100 mGy × 7 and 200 mGy × 7 groups at 24 and 48 h after the end of irra-diation compared with the control group.High-throughput sequencing showed that there were 882,475,and 1205 differen-tially expressed mRNAs in each dose group compared with the control group.GO analysis showed that the differentially ex-pressed mRNAs in each dose group were mainly enriched in biological processes such as cell cycle regulation,regulation of nitrogen compound metabolic process,regulation of cell division and response to stimulus.KEGG analysis showed that the differentially expressed mRNAs were mainly enriched in the pathways of cell cycle,cell senescence,and ferroptosis.Conclusion Fractional LDIR induced senescence in HBE cells,and differentially expressed mRNA-associated biological processes and pathways in senescent cells are related to cell cycle and cell senescence.
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