A fluorescent probe for visualization of hypochlorous acid and its application in environmental water samples
Hypochlorous acid(HOCl)is a residual disinfectant ingredient in environmental samples,especially in domestic water,which makes it difficult to achieve convenient detection.A probe(DHUOCl-C)that responds to HOCl has been developed from the classical dye methylene blue,in response to the problem of cumbersome methods for detecting HOCl.Nuclear magnetic resonance(NMR)hydrogen spectroscopy and high-resolution mass spectrometry(HRMS)show that the DHUOCl-C probe is successfully prepared.The performance tests such as fluorescence spectroscopy and absorption spectroscopy indicate that the probe is not only responsive(with a detection limit as low as 1.78 nmol/L),but also possesses a good selectivity.DHUOCl-C responds exclusively to HOCl,but not to other anions,cations and amino acids that may be present in ambient water samples.There is an obvious fluorescence change in response to HOCl,and 5 μmol/L of HOCl can cause the probe to undergo a significant color change that is visible to the eyes.In real environmental samples,the probe can identify and detect HOCl in water samples from different sources with the help of fluorescence or the color change of the system directly observed by the eyes,which demonstrates that the probe can meet the demand for convenient detection of HOCl in domestic water.
NETL
NSTL
万方数据
维普
