用于抑制肿瘤细胞生长的叶酸修饰聚多巴胺涂层的氧化铜纳米颗粒
Folic acid modified copper oxide nanoparticles coated with polydopamine for inhibition of tumor cell growth
邓凯夫 1郑轶璐 1王通 1郑之裕 1修梦婷 1任蓉 1VAN CUONG Nguyen 2朱利民1
作者信息
- 1. 东华大学生物与医学工程学院,上海;东华大学上海纳米生物材料与再生医学工程研究中心,上海
- 2. 胡志明市工业大学化学工程学院,胡志明市越南
- 折叠
摘要
采用沉淀转化法制备出粒径约为10 nm的氧化铜纳米颗粒(CuO NPs),通过盐酸多巴胺(DA HC1)在其表面进行聚合,并通过叶酸(FA)修饰形成CuO@PDA-FA NPs.采用透射电子显微镜、红外光谱仪、纳米粒径电势分析仪、X射线光电子能谱对纳米颗粒的形貌和性质进行表征.结果表明,制备的CuO@PDA-FA NPs粒径约为190 nm.材料降解亚甲基蓝试验和氧化葡萄糖试验证实CuO@PDA-FA NPs具备类过氧化物酶(POD)活性和类葡萄糖氧化酶(GOD)活性.近红外光热转换试验证实CuO@PDA-FA NPs具有良好的光热效应和光热稳定性.体外细胞毒性试验、细胞摄取试验和活性氧生成试验结果表明,CuO@PDA-FA NPs能主动靶向肿瘤细胞,并通过光热治疗(PTT)/化学动力学治疗(CDT)/饥饿治疗(ST)协同效果杀伤肿瘤细胞.
Abstract
Copper oxide nanoparticles(CuO NPs)with the size of about 10 nm were prepared by precipitation transformation method,and CuO@PDA-FA NPs were synthesized by polymerization of dopamine hydrochloride(DA HCl)and modification of folic acid(FA)on its surface.The morphology and properties of the nanoparticles were characterized by transmission electron microscope,infrared spectrometer,nano-particle size potential analyzer and X-ray photoelectron spectroscopy.The results show that CuO@PDA-FA NPs with a particle size of about 190 nm are prepared.The degradation of methylene blue and glucose oxide confirmed that the material has peroxidase(POD)activity and glucose oxidase(GOD)activity.Near-infrared photothermal conversion experiments show that it has good photothermal effect and photothermal stability.The results of in vitro cytotoxicity test,cell uptake test and reactive oxygen generation test show that CuO@PDA-FA NPs can actively target tumor cells and kill tumor cells through the synergistic effect of photothermal therapy(PTT),chemical dynamic therapy(CDT)and starvation therapy(ST).
关键词
氧化铜/纳米颗粒/叶酸/聚多巴胺/化学动力学治疗/光热治疗/模拟酶Key words
copper oxide/nanoparticle/folic acid/polydopamine/chemodynamic therapy/photothermal therapy/mimic enzyme引用本文复制引用
基金项目
上海市科委项目(22520710400)
上海市科委项目(21WZ2501300)
上海市科委项目(20DZ2254900)
中国教育部生物医用纺织材料"111工程"项目(B07024)
出版年
2024
NETL
NSTL
维普
万方数据