超声协同SDS-Na2CO3提取丝素蛋白及高强韧纳米纤维膜的制备与细胞相容性研究
Ultrasonic co-extraction of silk fibroin with SDS-Na2CO3 and preparation/cytocompatibility of high-strength and tough nanofiber membranes
牛佳杰 1寿万 2刘雍1
作者信息
- 1. 天津工业大学先进纺织复合材料教育部重点实验室,纺织科学与工程学院,天津
- 2. 阿肯色大学费耶特维尔分校机械工程系,阿肯色州美国
- 折叠
摘要
为解决提取过程中丝素蛋白极易受损的问题,提出以Na2CO3和表面活性剂SDS(十二烷基磺酸钠)的复合体系为脱胶剂,再结合超声处理的一体化天然蚕丝脱胶方案.采用该方案所得丝素蛋白纤维表面更加光洁平整,纤维损伤显著减少;相比传统的100 ℃碱液脱胶(30 min完全脱胶),该方案在75 ℃下仅用10 min即完成脱胶.以该方案所得脱胶纤维为原料,通过静电纺丝技术制备丝素蛋白纳米纤维膜,所得纤维膜经乙醇处理后,β折叠链片晶含量增至49.58％,展现出高达5.27 MPa的断裂应力和123.49％的断裂应变.在5 d的细胞培养试验中,丝素蛋白纳米纤维膜支持细胞的黏附、铺展和增殖,表现出良好的细胞相容性.这些研究成果将为制备高性能丝素蛋白纳米纤维膜提供解决方案,并有望推动丝素蛋白材料在生物医用领域的进一步应用和发展.
Abstract
Silk fibroin is highly susceptible to damage during the extraction process.To address this issue,this study proposes an integrated degumming solution that uses a composite system of Na2CO3 and the surfactant SDS(sodium dodecyl sulfate),combined with ultrasonic treatment.Silk fibroin fibers treated with this method exhibit a smoother and more even surface,with markedly less fiber damage.Compared to the traditional 100 ℃ alkaline degumming(30 min complete degumming),this scheme completes degumming in only 10 min at 75 ℃.Nanofibrous membranes were prepared from these degummed fibers using electrospinning technology,and their β-sheet crystalline content increased to 49.58％after ethanol treatment,along with high tensile strength(5.27 MPa)and elongation at break(123.49％).Over a 5-day cell culture period,the membranes have supported cell adhesion,spreading and proliferation,demonstrating excellent cytocompatibility.These research findings provide a novel solution for the production of high-performance silk fibroin nanofibrous membranes and are expected to advance the application and development of silk fibroin materials in the biomedical field.
关键词
丝素蛋白/脱胶/静电纺丝/细胞相容性Key words
silk fibroin/degumming/electrospinning/cytocompatibility引用本文复制引用
出版年
2024
NETL
NSTL
万方数据