Ultrasonic co-extraction of silk fibroin with SDS-Na2CO3 and preparation/cytocompatibility of high-strength and tough nanofiber membranes
Silk fibroin is highly susceptible to damage during the extraction process.To address this issue,this study proposes an integrated degumming solution that uses a composite system of Na2CO3 and the surfactant SDS(sodium dodecyl sulfate),combined with ultrasonic treatment.Silk fibroin fibers treated with this method exhibit a smoother and more even surface,with markedly less fiber damage.Compared to the traditional 100 ℃ alkaline degumming(30 min complete degumming),this scheme completes degumming in only 10 min at 75 ℃.Nanofibrous membranes were prepared from these degummed fibers using electrospinning technology,and their β-sheet crystalline content increased to 49.58％after ethanol treatment,along with high tensile strength(5.27 MPa)and elongation at break(123.49％).Over a 5-day cell culture period,the membranes have supported cell adhesion,spreading and proliferation,demonstrating excellent cytocompatibility.These research findings provide a novel solution for the production of high-performance silk fibroin nanofibrous membranes and are expected to advance the application and development of silk fibroin materials in the biomedical field.
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