In order to study the blocking effect of vegetables on nitrosamines,the inhibitory effect of perilla extract on endogenous nitrosamines was studied by combining liquid chromatography-mass spectrometry in a simulated gastric fluid environment.The results indicated that under simulated gastric fluid environment,perilla solution and sodium nitrite were added in simulated gastric juice environment,the sodium nitrite clearance rate could reach 10.4％.Perilla solution,sodium nitrite and diethylamine were added,LC-MS/MS was performed to detect the diethylnitrosamine production of blank sample and perilla added sample at a certain concentration were 0.2 ng/mL and 0.1 ng/mL,and the inhibition rate was 50％.NDMA,NDEA,NDBA and NPYR were significantly decreased in the diet supplemented with perilla.NDMA and NDEA were detected at 0.2 ng/g in the blank diet,but were not detected in the experimental group supplemented with perilla.NDBA blank control was 0.4 ng/g,the experimental group supplemented with perilla was 0.2 ng/g,the inhibition rate was 50％,NPYR blank control was 8.6 ng/g,the experimental group supplemented with perilla was 4.9 ng/g,the inhibition rate was 43％,to provide reference for studying the blocking mechanism of perilla on nitrosamine.Therefore,perilla had a significant blocking effect on endogenous nitrosamines.
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