首页|模拟胃液结合液质联用研究紫苏对内源性亚硝胺的阻断作用

模拟胃液结合液质联用研究紫苏对内源性亚硝胺的阻断作用

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为研究蔬菜对亚硝胺的阻断效果,在模拟胃液环境下结合液质联用,研究紫苏提取液对内源性亚硝胺的阻断作用.结果表明,在模拟胃液环境下加入紫苏液与亚硝酸钠,亚硝酸钠清除率可达10.4%.加入紫苏液、亚硝酸钠和二乙胺进行液相色谱质谱检测,空白样品和添加紫苏的样品中二乙基亚硝胺生成量分别为0.2、0.1 ng/mL,抑制率达到50%.添加紫苏的膳食中N-亚硝基二甲基(NDMA)、N-亚硝基二乙基(NDEA)、N-亚硝基二丁基(NDBA)、N-亚硝基吡咯烷(NPYR)含量显著降低.NDMA、NDEA在空白膳食中含量均为0.2 ng/g,紫苏试验组均未检出.NDBA空白对照为0.4 ng/g,紫苏试验组为0.2 ng/g,抑制率为50%;NPYR空白对照为8.6 ng/g,紫苏试验组为4.9 ng/g,抑制率为43%.因此,紫苏对内源性亚硝胺有明显的阻断作用.
Effect of Perilla in Blocking Endogenous Nitrosamines by Simulated Gastric Fluid Combined with Liquid Chromatography-Mass Spectrometry
In order to study the blocking effect of vegetables on nitrosamines,the inhibitory effect of perilla extract on endogenous nitrosamines was studied by combining liquid chromatography-mass spectrometry in a simulated gastric fluid environment.The results indicated that under simulated gastric fluid environment,perilla solution and sodium nitrite were added in simulated gastric juice environment,the sodium nitrite clearance rate could reach 10.4%.Perilla solution,sodium nitrite and diethylamine were added,LC-MS/MS was performed to detect the diethylnitrosamine production of blank sample and perilla added sample at a certain concentration were 0.2 ng/mL and 0.1 ng/mL,and the inhibition rate was 50%.NDMA,NDEA,NDBA and NPYR were significantly decreased in the diet supplemented with perilla.NDMA and NDEA were detected at 0.2 ng/g in the blank diet,but were not detected in the experimental group supplemented with perilla.NDBA blank control was 0.4 ng/g,the experimental group supplemented with perilla was 0.2 ng/g,the inhibition rate was 50%,NPYR blank control was 8.6 ng/g,the experimental group supplemented with perilla was 4.9 ng/g,the inhibition rate was 43%,to provide reference for studying the blocking mechanism of perilla on nitrosamine.Therefore,perilla had a significant blocking effect on endogenous nitrosamines.

Nitrosamineperillasimulated gastric fluidliquid chromatography-mass spectrometrysodium nitrite

喻凤香、陈煦、陈祖武、曾思景

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湖南生物机电职业技术学院,湖南长沙 410127

湖南省农业科学研究院,湖南长沙 410125

隆回县市场监督管理检验检测中心,湖南邵阳 422299

亚硝胺 紫苏 模拟胃液 液质联用 亚硝酸钠

湖南省自然科学基金

2023JJ60229

2024

中国果菜
中华全国供销合作总社济南果品研究院 山东省供销合作社联合社 中国果蔬贮藏加工技术研究中心

中国果菜

影响因子:0.204
ISSN:1008-1038
年,卷(期):2024.44(4)
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