Study on the Seed Transmission Mechanism of Tomato Mosaic Virus and Evaluation of the Dry Heat Treatment Effect
Tomato mosaic virus(ToMV)is a globally distributed virus that affects a wide range of hosts,with tomato being its primary host,significantly impacting tomato production.This study aimed to detect the localization of the virus in tomato seeds using RT-PCR.Additionally,it sought to assess the germination rate and ToMV inactivation rate following dry heat treatment,with the goal of identifying treatment conditions that effectively inactivate the virus while preserving the germination capacity of tomato seeds.ToMV was located in the seed coat of tomato seeds,with no detection in the endosperm or embryo.Compared with untreated tomato seeds,dry heat treatment at 75℃ for 48 h and 70℃ for 96 h significantly affected the germination of tomato seeds,while treatment at 80℃ for 24 h and treatment at 65℃ for 120 h did not significantly affect the germination rate.Constant temperature treatment at four different temperature effectively reduced the ToMV load in seeds,although the virus remained detectable by fluorescence quantitative PCR.Treatment at 80℃ for 24 h exhibited the most effective removal of ToMV without compromising the germination rate of tomato seeds.
万方数据
维普
