首页|甜瓜蔓枯病生物学特性及防治药剂筛选

甜瓜蔓枯病生物学特性及防治药剂筛选

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为明确甜瓜蔓枯病病原菌的生物学特性以及筛选出适合防治该病症的药剂,本试验在不同温度、pH、碳源和氮源条件下对该病原菌进行培养,确定对该病原菌生长最适温度、pH、碳源、氮源.采用菌丝生长速率法,用 3 种杀菌剂与PDA培养基混合制成含药培养基,测定抑制率,构建毒力回归方程并计算EC50.结果表明,甜瓜蔓枯病病原菌最适温度为 25℃,最适pH为6,最适碳源为葡萄糖,最适氮源为蛋白胨和硝酸钾.在供试的 3 种药剂中,百菌清对其抑制效果最好,EC50 为 2.097;其次为三唑酮,EC50 为 2.494;代森锰锌的作用效果最差,EC50 为 2.531.探讨不同环境条件对甜瓜蔓枯菌生长的影响,能够助力环保有效生物防治药剂的筛选,为甜瓜产业的可持续发展提供科学依据和技术支持.
Biological Characteristics of Melon Vine Blight and Screening of Preventive Agents
In order to clarify the biological characteristics of the pathogen of melon cran blight and select the drugs suitable for the prevention and treatment of the disease,this test cultured the pathogen under different temperature,pH,carbon source and nitrogen source conditions,and determined the most favorable temperature,pH,carbon source and nitrogen source for the growth of the pathogen.Using the mycelium growth rate method,three fungicides and PDA culture medium were mixed to make a drug-containing culture medium to determine the inhibition rate and build a virulence regression to calculate the EC50.The results showed that the most suitable temperature of melon blight pathogens was 25℃,the most suitable pH was 6,the most suitable carbon source was glucose,and the most suitable nitrogen source was protein and potassium nitrate.Among the three agents tested,Bamoxazole had the best inhibition effect,with EC50 of 2.097,followed by Triazolone,with EC50 of 2.494;and the worst effect of Daisen manganese zinc,with EC50 of 2.531.Exploring the effects of different environmental conditions on the growth of Fusarium graminearum could effectively assist in the screening of environmentally friendly and effective biological control agents,providing scientific basis and technical support for the sustainable development of the melon industry.

Melon vine blightsporulation quantitybiological characteristicsdrug screening

李洋、赵飞、邢鲲、邓洁玉、樊思莹、蒲秀兰、杨存第

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山西农业大学植物保护学院,山西 太原 030031

吕梁学院生物与食品工程系,山西 吕梁 033000

繁峙县农业产业发展中心,山西 忻州 034000

甜瓜蔓枯病 产孢量 生物学特性 药剂筛选

2024

中国果菜
中华全国供销合作总社济南果品研究院 山东省供销合作社联合社 中国果蔬贮藏加工技术研究中心

中国果菜

影响因子:0.204
ISSN:1008-1038
年,卷(期):2024.44(12)