Objective To observe the application effect of contrast-enhanced ultrasound(CEUS)in pre-operative examination of patients with complex anal fistula.Methods Total sixty-six patients with com-plex anal fistula admitted to our hospital from March 2020 to February 2023 were treated with ligation of the intersphincteric fistula tract(LIFT).They were divided into the control group(30 patients)and the observation group(36 patients).Patients in the control group underwent routine ultrasonography before operation,while patients in the observation group underwent CEUS.The operation time,hospital stay and blood loss of patients in the two groups were recorded.The diagnostic accuracy rates of of the internal o-pening,primary fistula,secondary fistula and abscess of patients in the two groups were compared,with the surgical diagnosis results as the gold standard.Results Operation time,hospital stay and blood loss were significantly shorter or less in the observation group than in the control group(P<0.05).The diag-nostic accuracy rates of the internal opening,primary fistula,secondary fistula and abscess were significant-ly higher in the observation group than in the control group(P<0.05).Conclusion CEUS has a high-er diagnostic accuracy rate of the internal opening and secondary fistula,etc.in the preoperative examina-tion of patients with complex anal fistula,and can reduce the operation time,intraoperative blood loss and hospital stay.