Objective To analyze the pathogenic bacteria distribution of incision infection after emergen-cy operation for acute intestinal obstruction and risk factors affecting incision infection.Methods A total of 140 patients with acute intestinal obstruction who underwent emergency operation at our hospital from April 2021 to August 2023 were selected as the research objects.The patients were divided into the infec-tion group and the non-infection group according to whether the postoperative wound was infected.The pathogenic bacteria in the infection group were detected by taking the purulent secretions from the incision to analyze the distribution of pathogenic bacteria.The independent risk factors of postoperative incision in-fection were analyzed by using the single factor analysis and Logistic regression analysis.Results A-mong 140 patients with acute intestinal obstruction who underwent emergency operation,27(19.29%)had postoperative incision infection.A total of 36 pathogenic strains were detected,mainly Escherichia coli and Klebsiella pneumoniae.The independent risk factors of postoperative incision infection in patients with acute intestinal obstruction after emergency operation were age ≥ 60 years,body mass index(BMI)≥28 kg/m2,diabetes mellitus,summer operation,operation time ≥120 min,class m incision,and mesen-teric vascular obstruction,P<0.05.Conclusion The incidence of postoperative incision infection after emergency operation for acute intestinal obstruction is high,and the pathogenic bacteria are mainly Esche-richia coli and Klebsiella pneumoniae.There are many risk factors for postoperative incision infection,and clinically,patients with old age,obesity,diabetes,summer operation,long operation time,class Ⅲ incision,and mesenteric vascular obstruction should be actively intervened.