Experimental Study on Therapeutic Effect of Zhuche Pill on TNBS Induced Crohn's Disease Rats
Objective To observe the therapeutic effect of Zhuche Pill on model rats with Crohn's disease(CD).Methods Total 40 SPF SD rats were randomly divided into blank control group,model control group,positive control group and Zhuche Pill treatment group,with 10 rats in each group.In addition to the blank control group,the model rats with CD were established by TNBS/ethanol induction in the other 3 groups.After the modeling was successful,the positive control group and the Zhuche Pill treatment group were given sulfasalazine(500 mg/kg)and Zhuche Pill suspension(2.835 g/kg)by intragastric ad-ministration,respectively,and the blank control group and the model control group were given the same amount of normal saline by intragastric administration.After continuous administration for 7 days,the therapeutic effect of Zhuche Pill on Crohn's disease was observed by evaluating disease activity index(DAI),colonmucosa damage index(CMDI)and histopathological score(HS).Results The DAI score of the model control group was higher than that of the blank control group within 7 days after modeling,and the difference was significant(P＜0.01).The DAI scores of the positive control group and the Zhuche Pill treatment group showed a decreasing trend with the prolongation of administration time,and the DAI scores of the two groups were significantly lower than that of the model control group from the third day of administration(P＜0.01).The CMDI and HS scores of the model control group were signifi-cantly higher than those of the blank control group(P＜0.01).After treatment,CMDI and HS scores in the positive control group and Zhuche Pill treatment group were significantly lower than those in the model control group(P＜0.01).Conclusion Zhuche Pill has a good therapeutic effect on rats with CD.
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