Network Pharmacology of Diyu(Sanguisorbae Radix)in Treatment of Inflammatory Bowel Disease
Objective To analyze the active components and target genes of Diyu(Sanguisorbae Radix)in the treatment of inflammatory bowel disease(IBD)based on network pharmacology.Methods Active components of Diyu were screened through TCMSP and ETCM databases,and the target genes were pre-dicted through TCMSP,Swiss Target Prediction,STITCH and PubChem databases.Target genes related to IBD were screened through DisGeNET,OMIM and GeneCards databases.Network analysis was performed using STRING database and Cytoscape 3.6.0 software.Gene ontology(GO)and signaling pathway enrich-ment were analyzed using DAVID 6.8 database.Results Total 14 active ingredients were obtained,in-cluding quercetin,kaempferol,betulinic acid,β-sitosterol,ellagic acid,gallic acid,daucosterol,etc.and 273 target genes.Total 213 IBD-related target genes were obtained.There are 62 key target genes in the treat-ment of IBD,including IL6,STAT3,VEGFA,TNF,AKT1,JUN,MAPK1,MMP9,CXCL8,INS and TP53,etc.These targets mainly exist in cytoplasm,nucleus,extracellular space,extracellular region,plasma mem-brane and other regions,which can combine with protein,enzyme,transcription factor activity,cytokine ac-tivity,growth factor activity,etc.,and be involved in transcriptional regulation,signal transduction,inflam-mation,apoptosis and immune response and other biological processes.It was associated with cancer path-way,PI3K-AKT,MAPK and other signaling pathways.Conclusion The active components of Diyu,in-cluding quercetin and kaempferol,can regulate cell apoptosis and inhibit cell inflammation through cancer pathway,PI3K-AKT and MAPK signal transduction.
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