Analysis of Intestinal Flora and Fecal Inflammatory Markers in Patients with Inflammatory Bowel Disease
Objective To analyze the changes of intestinal flora and fecal inflammatory markers in pa-tients with inflammatory bowel disease(IBD)and the differences of patients with different disease activi-ty.Methods Total 70 patients with IBD who were admitted to our hospital from March 2022 to Febru-ary 2024 were selected as study group,including 40 patients with ulcerative colitis(UC)and 30 patients with Crohn's disease(CD).Twenty healthy subjects who received colonoscopy at the same time were se-lected as control group.Fresh feces of all subjects were collected,and bacterial culture and inflammatory markers of fecal calcarein(FC),lactoferrin(LF),matrix metalloproteinase-9(MMP-9)and myeloperoxi-dase(MPO)were detected.The intestinal flora,levels of FC,LF,MMP-9 and MPO in the UC and CD pa-tients with different activity levels and the subjects in the control group were compared.Results Com-pared with the control group,the levels of enterobacter,enterococcus,yeast of aerobic bacteria and bacte-roides of anaerobic bacteria in the intestinal tract of IBD patients were significantly increased(P＜0.05).The levels of bifidobacterium,lactobacillus and eubacterium of anaerobic bacteria were significantly de-creased(P＜0.05),and the levels of staphylococcus of aerobic bacteria,peptococcus and clostridium of anaerobic bacteria were not significantly changed(P＞0.05).Compared with the control group,other flo-ra changes in UC and CD patients were consistent with the overall flora changes in IBD patients,except that the level of clostridium of anaerobic bacteria was increased in UC patients and decreased in CD patients(P＜0.05).With the increase of disease activity,the levels of enterobacter,enterococcus,yeast and bacte-roides of aerobic bacteria in UC and CD patients were increased successively(P＜0.05),while the levels of bifidobacterium,lactobacillus and eubacterium of anaerobic bacteria were decreased successively(P＜0.05).Compared with the control group,the levels of FC,LF,MMP-9 and MPO in IBD patients were sig-nificantly increased(P＜0.05),and the changes of FC,LF,MMP-9 and MPO in UC and CD patients were consistent with the overall changes in IBD patients.With the increase of disease activity,the levels of FC,LF,MMP-9 and MPO in UC and CD patients were increased successively(P＜0.05).Conclusion There are significant changes in intestinal flora and fecal inflammatory markers in patients with IBD,and there are some differences among patients with different disease activity,which is expected to provide refer-ence for the assessment of IBD activity.
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