首页|Bioprinting-Based High-Throughput Fabrication of Three-Dimensional MCF-7 Human Breast Cancer Cellular Spheroids

Bioprinting-Based High-Throughput Fabrication of Three-Dimensional MCF-7 Human Breast Cancer Cellular Spheroids

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Cellular spheroids serving as three-dimensional (3D) in vitro tissue models have attracted increasing interest for pathological study and drug-screening applications.Various methods,including microwells in particular,have been developed for engineering cellular spheroids.However,these methods usually suffer from either destructive molding operations or cell loss and non-uniform cell distribution among the wells due to two-step molding and cell seeding.We have developed a facile method that utilizes cellembedded hydrogel arrays as templates for concave well fabrication and in situ MCF-7 cellular spheroid formation on a chip.A custom-built bioprinting system was applied for the fabrication of sacrificial gelatin arrays and sequentially concave wells in a high-throughput,flexible,and controlled manner.The ability to achieve in situ cell seeding for cellular spheroid construction was demonstrated with the advantage of uniform cell seeding and the potential for programmed fabrication of tissue models on chips.The developed method holds great potential for applications in tissue engineering,regenerative medicine,and drug screening.

MCF-7 cellular spheroidsbioprintinghydrogelsconcave wellstissue on a chip

Kai Ling、Guoyou Huang、Juncong Liu、Xiaohui Zhang、Yufei Ma、Tianjian Lu、Feng Xu

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State Key Laboratory for Strength and Vibration of Mechanical Structures, School of Aerospace, Xi'an Jiaotong University, Xi'an 710049, China

Bioinspired Engineering and Biomechanics Center(BEBC), Xi'an Jiaotong University, Xi'an 710049, China

The Key Laboratory of Biomedical Information Engineering of the Ministry of Education.School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China

This work was financially supported by the National Natural Science Foundation of ChinaThis work was financially supported by the National Natural Science Foundation of ChinaThis work was financially supported by the National Natural Science Foundation of ChinaThis work was financially supported by the National Natural Science Foundation of ChinaChina Postdoctoral Science FoundationDoctoral Program of Higher Education of ChinaNatural Science Basic Research Plan in Shaanxi Province of ChinaFundamental Research Funds for the Central Universities

1137224311532009115222192013M540742201302011200712014JQ1004

2015

10.15302/J-ENG-2015062

工程科学(英文版)
中国工程院出版委员会

工程科学(英文版)

影响因子：0.226
ISSN：1672-4178
年,卷(期)：2015.1(2)
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