首页|Distinct immune escape and microenvironment between RG-like and pri-OPC-like glioma revealed by single-cell RNA-seq analysis

Distinct immune escape and microenvironment between RG-like and pri-OPC-like glioma revealed by single-cell RNA-seq analysis

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
The association of neurogenesis and gliogenesis with glioma remains unclear.By conducting single-cell RNA-seq analyses on 26 gliomas,we reported their classification into primitive oligodendrocyte precursor cell(pri-OPC)-like and radial glia(RG)-like tumors and validated it in a public cohort and TCGA glioma.The RG-like tumors exhibited wild-type isocitrate dehydrogenase and tended to carry EGFR mutations,and the pri-OPC-like ones were prone to carrying TP53 mutations.Tumor subclones only in pri-OPC-like tumors showed substantially down-regulated MHC-I genes,suggesting their distinct immune evasion programs.Furthermore,the two subgroups appeared to extensively modulate glioma-infiltrating lymphocytes in distinct manners.Some specific genes not expressed in normal immune cells were found in glioma-infiltrating lymphocytes.For example,glial/glioma stem cell markers OLIG1/PTPRZ1 and B cell-specific receptors IGLC2/IGKCwere expressed in pri-OPC-like and RG-like glioma-infiltrating lymphocytes,respectively.Their expression was positively correlated with those of immune checkpoint genes(e.g.,LGALS3)and poor survivals as validated by the increased expression of LGALS3 upon IGKC overexpression in Jurkat cells.This finding indicated a potential inhibitory role in tumor-infiltrating lymphocytes and could provide a new way of cancer immune evasion.

single-cell RNA-seqgliomaradial gliaprimitive oligodendrocyte precursor cellimmune escape

Weiwei Xian、Mohammad Asad、Shuai Wu、Zhixin Bai、Fengjiao Li、Junfeng Lu、Gaoyu Zu、Erin Brintnell、Hong Chen、Ying Mao、Guomin Zhou、Bo Liao、Jinsong Wu、Edwin Wang、Linya You

展开 >

Department of Human Anatomy & Histoembryology,School of Basic Medical Sciences,Fudan University,Shanghai 200032,China

Cumming School of Medicine,University of Calgary,Calgary,Alberta T2N 4N1,Canada

Glioma Surgery Division,Department of Neurosurgery,Huashan Hospital,Fudan University,Shanghai 200040,China

Department of Pathology,Huashan Hospital,Fudan University,Shanghai 200040,China

Department of Neurosurgery,Huashan Hospital,Fudan University,Shanghai 200040,China

Shanghai Key Laboratory of Medical Imaging Computing and Computer Assisted Intervention,Fudan University,Shanghai 200040,China

School of Mathematics and Statistics,Hainan Normal University,Haikou 570100,China

展开 >

talent startup funding from Fudan Universitytalent startup funding from Fudan Universitytalent startup funding from Fudan UniversityScience Innovation 2030-Brain Science and Brain-Inspired Intelligence Technology Major Project from the Ministry of Science and Science Innovation 2030-Brain Science and Brain-Inspired Intelligence Technology Major Project from the Ministry of Science and Shanghai Municipal Science and Technology Major ProjectZJ Lab operating grant of Shanghai Brain Bank technical systemAlberta Innovates Translational Chair Program in Cancer Genomics加拿大自然科学与工程研究委员会项目Canadian Foundation of Innovation

JIF101017SXF101012JIF1010472021ZD0201100 Task 42021ZD02011042018SHZDZX0116JC1420103RGPIN-2017-0488536655

2024

10.1007/s11684-023-1017-7

医学前沿
高等教育出版社

医学前沿

CSTPCD
影响因子：1.362
ISSN：2095-0217
年,卷(期)：2024.18(1)
  • 73