首页|MiRNA-451 is a potential biomarker for estrogenicity in mouse uterus
MiRNA-451 is a potential biomarker for estrogenicity in mouse uterus
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The uterotrophic assay has been commonly used to test environmental estrogens in vivo,however,it is often not sensitive enough sometimes.An alternative way is to evaluate estrogenicity through biomarker genes.MicroRNA (miRNA) is a class of regulatory gene,which has been shown to be a good biomarker for many diseases and toxicological effects in recent years,and some evidences showed that estrogen induced response was partially mediated by miRNAs.In this study,two types of microarrays were used to test the 17β-estradiol (E2) induced miRNA expression profile at different time points in the immature mouse uterus.Statistical analysis showed the aldehyde slide based array had less variation than the amino slide based array,and 11 dysregulated miRNAs were screened out for significant fold change.Real-time PCR was performed to further confirm that 4 out of 7 selected miRNAs,namely miR-451,miR-155,miR-335-5p,and miR-365,are E2 regulated miRNAs in the uterus.The function of the predicted targets of these miRNAs is involved in cell grow control,which is consistent with the main E2 function in the uterus.MiR-451 had similar strong responses to E2 in the uterus of both immature and overiectomized mice,and could be a potential biomarker for estrogenicity in the uterus.
estrogenmicroRNA (miRNA)microarraybiomarker
Lingyan HOU、Yun LU、Ying LI、Li LI
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College of Science, Beijing Forestry University, Beijing 100083, China
State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China
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