首页|Comparative analysis of DNA-SIP and magnetic-nanoparticle mediated isolation(MMI)on unraveling dimethoate degraders

Comparative analysis of DNA-SIP and magnetic-nanoparticle mediated isolation(MMI)on unraveling dimethoate degraders

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Microorganisms are crucial in the bioremediation of organophosphorus pesticides.However,most functional microorganisms(＞99％)are yet to be cultivated.This study applied two cultivation-independent approaches,DNA-SIP and magnetic-nanoparticle mediated isolation(MMI),to identify the functional microorganisms in degrading dimethoate in agricultural soils.MMI identified five dimethoate degraders:Pseudomonas,Bacillus,Ramlibacter,Arthrobacter,and Rhodococcus,whereas DNA-SIP identified three dimethoate degraders:Ramlibacter,Arthrobacter,and Rhodococcus.Also,MMI showed higher resolution than DNA-SIP in identifying functional microorganisms.Two organic phosphohydrolase(OPH)genes:ophC2 and ophB,were involved in dimethoate metabolism,as revealed by DNA-SIP and MMI.The degradation products of dimethoate include omethoate,O,O,S-trimethyl thiophosphorothioate,N-methyl-2-sulfanylacetamide,O,O-diethyl S-hydrogen phosphorodithioate,O,O,O-trimethyl thiophosphate,O,O,S-trimethyl thiophosphorodithioate,and O,O,O-trimethyl phosphoric.This study emphasizes the feasibility of using SIP and MMI to explore the functional dimethoate degraders,expanding our knowledge of microbial resources with cultivation-independent approaches.

Stable isotope probing(SIP)Magnetic-nanoparticle mediated isolation(MMI)DimethoateBiodegradationCultivation-independent approach

Luning Lian、Yi Xing、Dayi Zhang、Longfei Jiang、Mengke Song、Bo Jiang

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School of Energy and Environmental Engineering,University of Science & Technology Beijing,Beijing 100083,China

Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants,University of Science & Technology Beijing,Beijing 100083,China

National Environmental and Energy Science and Technology International Cooperation Base,University of Science & Technology Beijing,Beijing 100083,China

College of New Energy and Environment,Jilin University,Changchun 130021,China

Guangzhou Institute of Geochemistry,Chinese Academy of Sciences,Guangzhou 510640,China

College of Natural Resources and Environment,South China Agricultural University,Guangzhou 510642,China

National Engineering Laboratory for Site Remediation Technologies,Beijing 100015,China

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国家自然科学基金国家自然科学基金北京市自然科学基金中央高校基本科研业务费专项中央高校基本科研业务费专项Open Fund of National Engineering Laboratory for Site Remediation Technologies

42177359418071198212030FRF-TP-20-010A3FRF-IDRY-22-001NEL-SRT201907

2024

10.1007/s11783-024-1765-x

环境科学与工程前沿
高等教育出版社

环境科学与工程前沿

影响因子：0.545
ISSN：2095-2201
年,卷(期)：2024.18(1)
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