首页|Renewable biosynthesis of isoprene from wastewater through a synthetic biology approach:the role of individual organic compounds
Renewable biosynthesis of isoprene from wastewater through a synthetic biology approach:the role of individual organic compounds
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The biosynthesis of isoprene offers a more sustainable alternative to fossil fuel-based approaches,yet its success has been largely limited to pure organic compounds and the cost remains a challenge.This study proposes a waste-to-wealth strategy for isoprene biosynthesis utilizing genetically engineered E.coli bacteria to convert organic waste from real food wastewater.The impact of organic compounds present in wastewater on E.coli growth and isoprene production was systematically investigated.The results demonstrated that with filtration pretreatment of wastewater,isoprene yield,and production achieved 115 mg/g COD and 7.1 mg/(L·h),respectively.Moreover,even without pretreatment,isoprene yield only decreased by～24％,indicating promising scalability.Glucose,maltose,glycerol,and lactate are effective substrates for isoprene biosynthesis,whereas starch,protein,and acetate do not support E.coli growth.The optimum C/N ratio for isoprene production was found to be 8:1.Furthermore,augmenting essential nutrients in wastewater elevated the isoprene yield increased to 159 mg/g COD.The wastewater biosynthesis significantly reduced the cost(44％-53％decrease,p-value＜0.01)and CO2 emission(46％-55％decrease,p-value＜0.01)compared with both sugar fermentation and fossil fuel-based refining.This study introduced a more sustainable and economically viable approach to isoprene synthesis,offering an avenue for resource recovery from wastewater.
Min Yang、Xianehui Li、Weixiang Chao、Xiang Gao、Huan Wang、Lu Lu
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State Key Laboratory of Urban Water Resource and Environment,School of Civil and Environmental Engineering,Harbin Institute of Technology,Shenzhen 518055,China
Center for Materials Synthetic Biology,CAS Key Laboratory of Quantitative Engineering Biology of CAS,Shenzhen Institute of Synthetic Biology,Shenzhen Institutes of Advanced Technology,Chinese Academic of Science,Shenzhen 518000,China
Institute of Urban Ecology and Environmental Technology,School of Materials and Environmental Engineering,Shenzhen Polytechnic University,Shenzhen 518055,China
Shenzhen Science and Technology Program(China)Shenzhen Science and Technology Program(China)Shenzhen Science and Technology Program(China)国家自然科学基金State Key Laboratory of Urban Water Resource and Environment(Harbin Institute of Technology,China)广东省自然科学基金
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